Electrochemical sensor for multiplex biomarkers detection.
Authors:Wei F, Patel P, Liao W, Chaudhry K, Zhang L, Arellano-Garcia M, Hu S, Elashoff D, Zhou H, Shukla S, Shah F, Ho CM, Wong DT,
Journal:Clin Cancer Res
PubMed ID:19509137
'Multiplexing assay of biomarkers at the point-of-care is an elusive goal for molecular diagnostics. Here, we report an electrochemical (EC) sensor for oral cancer detection based on the simultaneous detection of two salivary biomarkers: interleukin (IL)-8 mRNA and IL-8 protein. Under the multiplexing mode, the limit of detection of salivary ... More
Size-based separations as an important discriminator in development of proximity ligation assays for protein or organism detection.
Authors:Leslie DC, Sohrabi A, Ikonomi P, McKee ML, Landers JP,
Journal:Electrophoresis
PubMed ID:20419707
'Proximity ligation is a powerful technique to measure minute concentrations of target protein with high specificity, and it has been demonstrated to be effective on a wide variety of protein targets. The proximity ligation assay (PLA) technique is shown to be compromised by the amplification of a nonspecific fluorescent product ... More
Eps homology domain endosomal transport proteins differentially localize to the neuromuscular junction.
Authors:Mate SE, Van Der Meulen JH, Arya P, Bhattacharyya S, Band H, Hoffman EP,
Journal:Skelet Muscle
PubMed ID:22974368
'Recycling of endosomes is important for trafficking and maintenance of proteins at the neuromuscular junction (NMJ). We have previously shown high expression of the endocytic recycling regulator Eps15 homology domain-containing (EHD)1 proteinin the Torpedo californica electric organ, a model tissue for investigating a cholinergic synapse. In this study, we investigated ... More
Unlike CD4+ T-cell help, CD28 costimulation is necessary for effective primary CD8+ T-cell influenza-specific immunity.
Authors:Seah SG, Carrington EM, Ng WC, Belz GT, Brady JL, Sutherland RM, Hancock MS, La Gruta NL, Brown LE, Turner SJ, Zhan Y, Lew AM,
Journal:Eur J Immunol
PubMed ID:22585421
The importance of costimulation on CD4(+) T cells has been well documented. However, primary CTLs against many infections including influenza can be generated in the absence of CD4(+) T-cell help. The role of costimulation under such ... More
Enzymatic route to preparative-scale synthesis of UDP-GlcNAc/GalNAc, their analogues and GDP-fucose.
Authors:Zhao G, Guan W, Cai L, Wang PG,
Journal:Nat Protoc
PubMed ID:20224564
Enzymatic synthesis using glycosyltransferases is a powerful approach to building polysaccharides with high efficiency and selectivity. Sugar nucleotides are fundamental donor molecules in enzymatic glycosylation reactions by Leloir-type glycosyltransferases. The applications of these donors are restricted by their limited availability. In this protocol, N-acetylglucosamine (GlcNAc)/N-acetylgalactosamine (GalNAc) are phosphorylated by N-acetylhexosamine ... More