Thermo Scientific AminoLink Plus Micro固定化キットは、抗体精製や他のアフィニティープロトコルで使用するアミン含有リガンドの共有結合的付加を可能にします。AminoLink Plus Micro固定化キットの特長:•少量使用に最適化—粗血清から特異的抗体を精製するために、少量詳細を見る
How do I know whether to use pH 7.2 or pH 10 for coupling my protein to AminoLink Plus Gel?
Coupling at pH 10 generally gives some increase in efficiency but may damage some ligands and requires a more involved procedure. If stability of the ligand is in question or the increase in coupling efficiency is of minor importance, use the pH 7.2 coupling procedure. This procedure has fewer steps and will still give good coupling efficiency.
What is the difference between AminoLink Coupling Resin and NHS-Activated Agarose?
Both react with primary amines, but AminoLink Coupling Resin is a two-step conjugation. First a Schiff base is formed between the amine and the aldehyde on the resin, which is then reduced to a stable secondary amine with sodium cyanoborohydride, whereas NHS-Activated Agarose utilizes an NHS ester to form an amide bond in a one-step conjugation.
What is the difference between AminoLink and AminoLink Plus supports?
AminoLink Plus Coupling Resin is activated at a higher level and has higher flow rates than the original AminoLink Coupling Resin, resulting in higher capacity and faster purification. AminoLink Supports can be used to immobilize any molecule with a primary amine.
How do your AminoLink Coupling Resin and AminoLink Plus Coupling Resin work?
AminoLink and AminoLink Plus Supports are activated with aldehyde groups which will react with primary amines to form Schiff bases, which are reduced to stable, non-reversible secondary amines. Coupling efficiency often exceeds 85% with this support.