Pierce™ DSG, No-Weigh™ Format

Citations & References (6)

Thermo Scientific™

Pierce™ DSG, No-Weigh™ Format

Name: Pierce™ DSG, No-Weigh™ Format
SKU: 20593

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製品番号（カタログ番号）	数量
20593	50 mg
A35392	10 x 1 mg

2 オプション

製品番号（カタログ番号） 20593

価格（JPY）

28,600

Each

お問い合わせください ›

数量:

50 mg

一括またはカスタム形式をリクエストする

Thermo Scientific Pierce DSGは水溶性のホモ二官能性N-ヒドロキシスクシンイミドエステル（NHSエステル）クロスリンカーで、放射性標識リガンドを細胞表面受容体に結合させるためによく使用されます。NHSエステルは、タンパク質やペプチドのクロスリンクと標識にもっとも一般的に使用される、もっともシンプルな反応基です。NHSエステルは、ペプチドのN末端にある一級アミンおよびリジン残基のアミンと反応して、安定したアミド共役結合を形成し、NHS基を放出します。

Thermo Scientific No-Weigh製品は、分注済みフォーマットで提供される特殊試薬です。計量済みのパッケージではバイアルを繰り返し開閉することがないため、経時的な試薬反応性の喪失や汚染を防ぎます。このフォーマットでは、毎回新しい試薬バイアルを使用できるため、少量の試薬を計量する手間がなくなり、試薬の安定性に対する懸念が軽減します。

グルタル酸ジスクシンイミジルの特長：

•反応基：NHSエステル（両端）
• 反応の対象：アミノ基（一級アミン）
• 非切断性
• 水溶性（まずDMFまたはDMSOに溶解）
• 膜透過性であるため、細胞内クロスリンクが可能
• 一部のアプリケーションでは、DSSと比較してクロスリンク効率を向上

製品リファレンス
クロスリンカーアプリケーションガイド -- この製品に関する最近の参考文献情報を検索できます

研究用途にのみご使用ください。診断目的には使用できません。

仕様

細胞透過性Yes

形状Powder

標識法化学的標識

分子量326.26

PEG化No

製品ラインPierce

数量50 mg

反応性部分NHSエステル

出荷条件Ambient

溶解性DMF、DMSO

スペーサーアーム長7.7 Å

水溶性No

化学反応性Amine-Amine

CleavableNo

クロスリンカータイプホモ二官能性

フォーマットStandard, Single-use

製品タイプ架橋剤

スペーサー短鎖

Unit SizeEach

組成および保存条件

4℃で乾燥した場所に保存。室温で出荷

よくあるご質問（FAQ）

Can you provide the shelf-life for DSG (disuccinimidyl glutarate)?

DSG (disuccinimidyl glutarate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How should I dissolve and store DSG, No-Weigh Format?

DSG is an NHS-ester that hydrolyzes easily and is moisture-sensitive. For optimal results, we recommend storing DSG powder desiccated at 4 degrees C. To avoid moisture condensation onto the product, the vial must be equilibrated to room temperature before opening. We recommend resuspending DSG in DMSO, Anhydrous (Cat. No. D12345) right before use and not storing the solution.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can NHS-diazirine (Cat. No. 26167) be used for CHIP applications?

NHS-diazirine has not been tested for cross-linking in a ChIP application. EGS (Cat. No. 21565) and DSG (Cat. No. 20593) are not used by themselves in ChIP, but rather in combination with formaldehyde. While formaldehyde is good for crosslinking proteins that are in direct contact with DNA, it cannot trap proteins that may be bound in a complex with other proteins but are not in direct contact. Thus EGS or DSG can cross-link proteins to proteins that are in direct contact with DNA and are crosslinked to it by formaldehyde. See this reference for more information

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

引用および参考文献 (6)

引用および参考文献

Abstract

The structural basis for the oligomerization of the N-terminal domain of SATB1.

Authors:Wang Z, Yang X, Chu X, Zhang J, Zhou H, Shen Y, Long J

Journal:Nucleic Acids Res

PubMed ID:22241778

Special AT-rich sequence-binding protein 1 (SATB1) is a global chromatin organizer and gene expression regulator essential for T-cell development and breast cancer tumor growth and metastasis. The oligomerization of the N-terminal domain of SATB1 is critical for its biological function. We determined the crystal structure of the N-terminal domain of ... More

Neurotrophin-mediated degradation of histone methyltransferase by S-nitrosylation cascade regulates neuronal differentiation.

Authors:Sen N, Snyder SH

Journal:Proc Natl Acad Sci U S A

PubMed ID:22123949

Epigenetic regulation of histones mediates neurotrophin actions with histone acetylation enhancing cAMP response element-binding (CREB)-associated transcription elicited by brain-derived neurotrophic factor (BDNF) and nerve-growth factor (NGF). Roles for histone methylation in CREB's transcriptional activity have not been well characterized. We show that depletion of the histone methyltransferase suppressor of variegation ... More

Characterizing phospholamban to sarco(endo)plasmic reticulum Ca2+-ATPase 2a (SERCA2a) protein binding interactions in human cardiac sarcoplasmic reticulum vesicles using chemical cross-linking.

Authors:Akin BL, Jones LR

Journal:J Biol Chem

PubMed ID:22247554

Chemical cross-linking was used to study protein binding interactions between native phospholamban (PLB) and SERCA2a in sarcoplasmic reticulum (SR) vesicles prepared from normal and failed human hearts. Lys(27) of PLB was cross-linked to the Ca(2+) pump at the cytoplasmic extension of M4 (at or near Lys(328)) with the homobifunctional cross-linker, ... More

A central role for the T1 domain in voltage-gated potassium channel formation and function.

Authors:Strang C, Cushman SJ, DeRubeis D, Peterson D, Pfaffinger PJ

Journal:J Biol Chem

PubMed ID:11312262

To interpret the recent atomic structures of the Kv (voltage-dependent potassium) channel T1 domain in a functional context, we must understand both how the T1 domain is integrated into the full-length functional channel protein and what functional roles the T1 domain governs. The T1 domain clearly plays a role in ... More

RelA Ser276 phosphorylation-coupled Lys310 acetylation controls transcriptional elongation of inflammatory cytokines in respiratory syncytial virus infection.

Authors:Brasier AR, Tian B, Jamaluddin M, Kalita MK, Garofalo RP, Lu M

Journal:J Virol

PubMed ID:21900162

Respiratory syncytial virus (RSV) is a negative-sense single-stranded RNA virus responsible for lower respiratory tract infections (LRTIs) in humans. In experimental models of RSV LRTI, the actions of the nuclear factor ?B (NF-?B) transcription factor mediate inflammation and pathology. We have shown that RSV replication induces a mitogen-and-stress-related kinase 1 ... More

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