Can you provide the shelf-life for Sulfo-SIAB (sulfosuccinimidyl (4-iodoacetyl)aminobenzoate)?
Sulfo-SIAB (sulfosuccinimidyl (4-iodoacetyl)aminobenzoate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides ... More
Activation of alpha-tropomyosin exon 2 is regulated by the SR protein 9G8 and heterogeneous nuclear ribonucleoproteins H and F.
Authors:Crawford JB, Patton JG
Journal:Mol Cell Biol
PubMed ID:17000773
'The inclusion of exons 2 and 3 of alpha-tropomyosin is governed through tissue-specific alternative splicing. These exons are mutually exclusive, with exon 2 included in smooth muscle cells and exon 3 included in nearly all other cell types. Several cis-acting sequences contribute to this splicing decision: the branchpoints and pyrimidine ... More
A high-capacity RNA affinity column for the purification of human IRP1 and IRP2 overexpressed in Pichia pastoris.
Authors:Allerson CR, Martinez A, Yikilmaz E, Rouault TA
Journal:RNA
PubMed ID:12592010
'Regulated expression of proteins involved in mammalian iron metabolism is achieved in part through the interaction of the iron regulatory proteins IRP1 and IRP2 with highly conserved RNA stem-loop structures, known as iron-responsive elements (IREs), that are located within the 5'' or 3'' untranslated regions of regulated transcripts. As part ... More
Miniature single-particle immunoassay for prostate-specific antigen in serum using recombinant Fab fragments.
Authors:Härmä H, Tarkkinen P, Soukka T, Lövgren T
Journal:Clin Chem
PubMed ID:11067810
Quantitative, miniaturized nucleic acid assays and immunoassays can be developed with single microparticles, microfluorometric detection, and intrinsically fluorescent lanthanide chelates in a multiple assay format to decrease reagent consumption, cost, and assay time. We used recombinant Fab fragments to capture and detect free and total prostate-specific antigen (PSA) from serum ... More