Pierce™ 6xHis Protein Tag Stain Reagent Set
Pierce™ 6xHis Protein Tag Stain Reagent Set
Thermo Scientific™

Pierce™ 6xHis Protein Tag Stain Reagent Set

Thermo Scientific Pierce 6xHisタンパク質タグ染色試薬セットは、タンパク質ポリアクリルアミドゲル中のヒスチジンタグ付き(Hisタグ付き)組換え融合タンパク質を優先的に検出するための蛍光染色です。独自の蛍光染色剤は、ポリアクリルアミドゲル(SDS-PAGEなど詳細を見る
製品番号(カタログ番号)数量
245701 L
製品番号(カタログ番号) 24570
価格(JPY)
84,100
Each
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数量:
1 L
一括またはカスタム形式をリクエストする
Thermo Scientific Pierce 6xHisタンパク質タグ染色試薬セットは、タンパク質ポリアクリルアミドゲル中のヒスチジンタグ付き(Hisタグ付き)組換え融合タンパク質を優先的に検出するための蛍光染色です。

独自の蛍光染色剤は、ポリアクリルアミドゲル(SDS-PAGEなど)で電気泳動されたヒスチジンタグ融合タンパク質を特異的に検出します。この染色剤を使用すると、Hisタグ付き組換えタンパク質の発現を直接ゲル内で評価できるため、Hisタグ付きタンパク質の純度と収量を確認するために一般的に使用される膜転写やウェスタンブロッティングのステップが不要になります。検出下限は、使用するUVランプ (300 nm) と検出装置 (CCD カメラが最良) に依存しますが、本染色剤はHisタグ融合タンパク質生産の進行状況の評価に有用であり、その後、Coomassie染色や他の総タンパク質染色を行うことができます。

6xHisタンパク質タグ染色試薬セットの特長:

ウェスタンブロッティングより2~3倍高速— 迅速検出が可能で貴重な実験時間を節約
ゲル上で直接検出— 0.2 µg以上の6xHisタグ付きタンパク質を検出するための膜転写およびウエスタンブロットステップが不要になります
すぐに使用可能、2剤式—混合不要、希釈不要、手間がかからず、簡単な実行とミスのない検出を保証します
6xHisタグ付きタンパク質の特異的蛍光検出—目的のタンパク質のみを検出します(少量のタンパク質にはCCDカメラ、多量のタンパク質にはUVトランスイルミネーターを推奨)
二重染色に対応—Hisタグ付きタンパク質をThermo Scientific GelCodeブルー染色試薬で染色して、タンパク質のプロファイルを決定します

以下を含みます:
• 染色剤および現像液(各500 mL)

以下が必要です:
• UVランプ光源(300 nm)およびCCDカメラ
研究用途にのみご使用ください。診断目的には使用できません。
仕様
概要Pierce 6xHisタンパク質タグ染色試薬キット
検出位置ゲル内検出
検出法蛍光
製品ラインPierce
製品タイプHisタグタンパク質染色セット
数量1 L
標的分子タンパク質(Hisタグ付き)
標識または色素独自のミックス
Unit SizeEach
組成および保存条件
コンポーネントは室温で保管してください。製品は室温で出荷されます

よくあるご質問(FAQ)

I used the Thermo Scientific 6xHis Protein Tag Stain Reagent Set to detect my 6xHis-tagged protein but am also detecting non-tagged proteins. Why is this?

This is likely due to weak cross-reaction staining of proteins containing histidine clusters. Here are our recommendations:

- Wash gel for additional time in water (step 5.)
- Slightly decrease staining time (step 2.)
- Adjust exposure time and other settings to minimize weak, non-specific staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

With the Thermo Scientific 6xHis Protein Tag Stain Reagent Set, I was able to detect the positive control but not the experimental protein. What happened?

Here are possible causes and solutions:

- Experimental protein not expressed at sufficient levels in the lysate being tested. Load more lysate per lane or otherwise check that the target protein is expressed at all.
- Experimental recombinant protein is not tagged with 6xHis. Check for presence of tag by an independent method (e.g., detection or purification by nickel-chelate chemistry.
- 6xHis tag on experimental protein is blocked by interfering substances in sample. Verify that nickel and other 6xHis-binding reagents were not brought forward from a previous step and use only high-quality water.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the Thermo Scientific 6xHis Protein Tag Stain Reagent Set but was not able to detect any bands for either the positive control or experimental protein. What possibly went wrong?

Here are possible causes and solutions:

- Poor quality or insufficient exposure to appropriate UV-light source. If possible, use a CCD camera for detection; ensure that UV lamp delivers the appropriate wavelength for excitation (280-310 nm).
- Experimental protein is poorly expressed (insufficient loading). Insufficient protein was electrophoresed per lane for the detection method used.
- Insufficient washing; residual SDS in gel prevents binding of stain. Wash gel for 3 × 20 minutes in ultrapure water and restain .
- Experimental protein is small (less than 20kDa) and diffused from gel during washing step. Fix the gel 50% methanol:7% acetic acid for 15 minutes before performing the water wash.
- Poor diffusion of stain into gel. Increase staining time to 10 minutes (step 2); this may be repeated on the same gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

With the Thermo Scientific 6xHis Protein Tag Stain Reagent Set, how long will the fluorescent signal remain stable after the gel is stained'?

The fluorescent signal is stable for several hours in gels stored in water. Signal may be detectable, if somewhat attenuated, after overnight storage.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is there a fixation step in the Thermo Scientific 6xHis Protein Tag Stain Reagent Set staining procedure?

There is no fixation step in the Thermo Scientific 6xHis Protein Tag Stain Reagent Set staining procedure. Hence, staining with this kit does not inhibit subsequent total protein staining with general protein stains, or electrophoretic transfer to membrane. Note: Bis-Tris gels run in MOPS or MES buffer may require fixing in 50% methanol: 7% acetic acid for 15 minutes before performing the stain procedure. After electrophoresis, fix the gel and then proceed with Step 1 of the procedure.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.