対応範囲:60のアイソタイプ測定(検査サンプルあたり8ウェル) • コーティング済み96ウェルアイソタイピングプレート、5枚 • ヤギ抗マウスIgA+IgA-IgM-HRPコンジュゲート、30 mL • TMB基質、55 mL • 停止液、55 mL • 30倍洗浄バッファー、200 mL • TBSバッファーパック(各500 mL)、2パック
4℃で保管してください。
よくあるご質問(FAQ)
How does the Rapid ELISA Mouse Antibody Isotyping kit (Cat. No. 37503) differ from the Cassette-based Kits (Cat. Nos. 26178 and 26179)?
The ELISA Kit procedure takes approximately 1.5 hours to complete and enables simultaneous testing of multiple samples (12 per plate). The Cassette-based Kits provide results in only 5 minutes but are single-use units that are less practical and more costly per sample than the ELISA Kit. The Cassette-based Kits are less sensitive (minimum antibody concentration is 10 ng/mL) and provide only qualitative assessment.
What if I see cross-reactivity for kappa light and IgG3 and IgG2 using the Rapid ELISA Mouse Antibody Isotyping kit?
Try diluting samples. Some antibodies have epitopes that cross-react with the IgG3 and IgG2 antibodies used in the kit; when the sample dilution is optimized, this cross-reaction will produce significantly less signal (color) compared to the true identity.
How do I evaluate results using the Rapid ELISA Mouse Antibody Isotyping kit?
The assay can be evaluated qualitatively by visual inspection or quantitatively by measuring absorbances in a spectrophotometer (plate reader) at 450 nm.
I only need to test two samples using the Rapid ELISA Mouse Antibody Isotyping kit. What can I do with the other sample strips in the plate frame so that they can be used later?
The supplied plates are strip-plates, meaning that individual 8-well strips (columns) can be removed from the plate frame. To test two samples, remove 10 strips, leaving two strips in the plate frame for testing. Place unused strips in the foil pouch with the provided desiccant. Store reserved strips at 2-8°C. Be sure the foil pouch is tightly sealed. After completing the assay, retain the plate frame for use with reserved strips for future assays.