Thermo Scientific™

Pierce™ Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse

Catalog number: 26179
Thermo Scientific™

Pierce™ Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse

Catalog number: 26179
Catalog Number
26179
Unit Size
10 test
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2617910 test
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
The Thermo Scientific Pierce Rapid Mouse Antibody Isotyping Kit Plus Kappa and Lambda is a single-use, cassette-based kit for 5-minute lateral-flow assay determination of mouse monoclonal antibody class and subclass identity.

This antibody isotyping kit uses cartridges that provide a color-readout of the monoclonal antibody isotype within five minutes after pipetting a small amount of diluted culture supernatant or ascites fluid sample into the sample well. The kit determines mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, as well as light-chain identity (kappa vs. lambda light chains).

Features of the Pierce Rapid Mouse Antibody Isotyping Kit:

Long shelf life—stable for at least one year at room temperature
Single-step—add diluted antibody sample to the loading-well of cassette
Fast—within 5 minutes a band appears that indicates the antibody isotype
Sensitive—detect antibodies in tissue culture media or ascites fluid samples at any concentration greater than 10 ng/mL
Specific—assay does not cross-react with fetal bovine serum (FBS)
Reliable—results are similar to the standard ELISA-based isotyping assays

Each Pierce Rapid Isotyping Kit assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the small cassette. Gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.

Determining the class and subclass of a monoclonal antibody is useful in planning the best immunoglobulin purification method. For example, mouse IgA and IgM are best purified by size (i.e., gel exclusion) or using immunoaffinity separation columns. Mouse IgG2a and IgG2b are purified with immobilized Protein A at pH 7-8, while Mouse IgG1 binds best to Protein A at pH 8-9. Immunoglobulin that contains kappa light chains can be purified using immobilized Protein L.

The Pierce Rapid Isotyping Kit is compatible with both tissue culture supernatant and mouse ascites fluid, and is more sensitive than conventional latex-bead based dipstick assays and much faster than ELISA-based isotyping assays. The kit requires only 0.5μL ascites fluid, 5 to 55μL of cell culture supernatant or 1.5ng of purified antibody.

Related Products
Rapid ELISA Mouse mAb Isotyping Kit
Pierce™ Rapid Antibody Isotyping Kit - Mouse
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Description
Pierce Rapid Isotyping Kit plus Kappa and Lambda - Mouse
For Use With (Application)
Membrane Blot
Quantity
10 Tests
Assay
Isotyping Assay
Detection Method
Chromogenic
Product Line
Pierce™
Target Specificity
Target-specific

Contents & Storage

Sufficient For: 10 isotype determinations
• Isotyping Cassettes (three per pouch), 10 pouches
• Sample Diluent, 45 mL

Store kit at 2–8°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text