Rapid ELISA Mouse mAb Isotyping Kit

Catalog number: 37503

Thermo Scientific™  Related applications: Antibody Production

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Description

The Thermo Scientific Pierce Rapid ELISA Mouse mAb Isotyping Kit provides antigen-independent determination of mouse monoclonal antibody isotype using a microplate method that enables multiple samples to be tested in about one hour.

Features of the Rapid ELISA Mouse mAb Isotyping Kit:

Fast—determine antibody isotype in approx. 1 hour; considerably faster than traditional ELISA kits
Convenient—eight-well strip format allows partial use of the plate; use one strip (column) for each sample (12 samples per plate)
Specific—characterize antibodies for six different subclasses and two different light-chain types
Sensitive—accurate characterization with samples containing at least 3 ng/mL of test antibody
Flexible—kit is compatible with hybridoma cell culture supernatant, ascites fluid or purified antibodies
Cost effective—cost per assay is less than with single-use assay strips or cassettes
No special equipment needed—assess results visually or measure quantitatively using an ordinary ELISA plate reader (450nm)
Complete and easy to use—includes precoated plates, detecting antibody, buffers, and TMB substrate and stop solutions

This fast assay uses ELISA strip-plates that are pre-coated in different wells with anti-mouse heavy-chain capture antibodies (anti-IgG1, IgG2a, IgG2b, IgG3, IgA and IgM) or anti-mouse light-chain capture antibody (kappa or lambda). This approach eliminates the need to purify and immobilize an antigen to determine immunoglobulin isotype (antibody subclass and light chain identity). A mouse monoclonal antibody sample applied to the wells can be isotyped within one hour. Results are evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450nm.

Related Products
Pierce™ Rapid Antibody Isotyping Kit - Mouse
Pierce™ Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Absorbance, ELISA (Antigen Capture)
Assay Type: Isotyping Assay
Product Size: 60 test kit
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader

Contents & storage

Sufficient For: 60 isotype determinations (8 wells per test sample)
• Pre-coated 96-well Isotyping Plates, 5 plates
• Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30 mL
• TMB Substrate, 55 mL
• Stop Solution, 55 mL
• 30X Wash Buffer, 200 mL
• TBS Buffer Packs (each makes 500 mL), 2 packs

Store at 4°C.

Documents

Manuals & protocols