MicroSEQ™ Full Gene 16S rDNA PCR Kit
Product Image
Applied Biosystems™

MicroSEQ™ Full Gene 16S rDNA PCR Kit

The MicroSEQ™ Full Gene 16S rDNA PCR Kit is the PCR component of the MicroSEQ™ 16S rDNA Full Gene Identification詳細を見る
製品番号(カタログ番号)数量
43491551 Kit
製品番号(カタログ番号) 4349155
価格(JPY)
102,300
Each
お問い合わせください ›
数量:
1 Kit

The MicroSEQ™ Full Gene 16S rDNA PCR Kit is the PCR component of the MicroSEQ™ 16S rDNA Full Gene Identification Kit, which provides an easy-to-use DNA sequence-based method to identify most bacteria. Use the PCR kit to amplify the bacterial 16S rRNA gene, for subsequent sequencing using the MicroSEQ™ Full Gene 16S rDNA Sequencing Kit. This PCR kit does not include a protocol or a Quick Reference Card.

  • Sequence-based measurement ensures you get reproducible results in under 24 hours.
  • Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
  • Universal domain-level primers eliminate up-front optimization.
  • MicroSEQ™ ID Analysis Software ensures accurate species identification.
  • Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
  • Requires minimal training-similar work flow as the MicroSEQ™ D2 LSU fungal kit and the MicroSeq 500 16S rDNA kit.

The MicroSEQ™ ID System: A Complete, Integrated Solution

Streamlining every step in microbial identification, Applied Biosystems™ MicroSEQ™ System combines the advantages of MicroSEQ™ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ™ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)GeneAmp 9700, Veriti Thermal Cycler
ライブラリCustomizable, Bacterial Library (> 2000 species)
製品ラインMicroSEQ
製品タイプFull Gene 16S rDNA PCR Kit
数量1 Kit
サンプルタイプDNA (Genomic)
出荷条件ドライアイス
ソフトウェアMicroSEQ™ ID Analysis Software
ターゲット16S rRNA (full gene)
対象微生物クラスBacteria
技術SBT (Sequence-Based Typing)
検出法Primer-probe
使用対象(アプリケーション)Standard PCR
GC-Rich PCR Performance
Unit SizeEach
組成および保存条件
• 2X PCR Master Mix (sufficient for 20 amplifications), 3 tubes
• Positive Control DNA, 1 tube
• Negative Control DNA (water), 1 tube
• Protocol and Quick Reference Guide

After first use, store at 2°C to 8°C in a PCR clean room.

よくあるご質問(FAQ)

What primers are supplied with the MicroSEQ Full Gene 16S rDNA PCR Kit?

The MicroSEQ Full Gene 16S rDNA PCR Kit includes universal domain-level primers that are designed to amplify the bacterial 16S rRNA gene.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

Where can I get a copy of the free trial version of the MicrobeBridge Software?

A 60-day-free trial of the MicrobeBridge Software can be downloaded here: www.thermofisher.com/order/catalog/product/A29238

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.

Who do I contact for support for MicrobeBridge Software and for MicrobeNet database issues?

For the MicrobeBridge Software, please contact your local Thermo Fisher Scientific Technical Support (https://www.thermofisher.com/us/en/home/technical-resources/contact-us.html) or email abtechsupport@thermofisher.com.

For MicrobeNet, please contact the CDC directly at microbenet@cdc.gov. This account is actively monitored by the CDC MicrobeNet staff.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.

What are the minimum system requirements for installing the MicrobeBridge Software?

The MicrobeBridge Software has the following recommended hardware requirements: Minimum 1.8 GHz processor, 2 GB RAM, and 20 GB of free hard disk space. MicrobeBridge Software has been tested on Windows 7 32-bit SP1, Windows 7 64-bit SP1, and Windows 10 IoT.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.

Will I lose any data if I upgrade from the MicrobeBridge Software free trial version to the full version?

No data is lost with the MicrobeBridge Software installation of the full version on top of the free trial version.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.