Melon™ Gel Monoclonal IgG Purification Kit

For serum, a volume double the Melon Gel Support volume is the maximum amount that can be processed at one time. To determine if too much sample was processed, examine the purified IgG by SDS-PAGE for additional protein bands.

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No. Thermo Scientific Thiophilic Adsorbent (Cat. Nos. 20500 and 44916) can be used to purify IgY from serum. The Chicken IgY Purification Kit (Cat. No. 44918) can be used to purify IgY from egg yolks.

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Yes. With the Melon Gel IgG Spin Purification Kit, 90% recovery of IgG with greater than 80% purity are typically observed for a single purification. Although IgA and IgM may be present along with the purified IgG, a high percentage (> 80%) of IgA and IgM is retained on the gel support. This high binding of IgA and IgM to the gel support combined with the fact that IgA and IgM are present in low abundance in serum results in low levels of these antibody molecules in the purified IgG. Here are other advantages of using the Melon Gel IgG Spin Purification Kit over traditional Protein A or Protein G methods:
-Simple, one-step protocol - IgG purification with Melon Gel Resin does not involve binding, washing, and multiple elution steps required by Protein A and Protein G
-Rapid purification - antibody purification from serum is four to six times faster than Protein A or G methods
-Minimal dilution - reduced need to concentrate antibody before storage or use
-Robust purification - Melon Gel IgG Purification Kits work with a wide range of antibodies including many that do not purify well on Protein A or Protein G
-Gentle purification - No harsh antibody elution conditions helps retain IgG activity
-Regenerable support - Melon Gel Resin can be used for multiple antibody purifications

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The Melon Gel Support can be used up to three times. Performing an ammonium sulfate precipitation on serum samples containing large amounts of non-antibody protein before purification improves purification efficiency and increases reusability.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

No. However, researchers may want to add an equal volume of binding buffer to elevate the pH towards neutral.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.