Axiom™ OVSNP600 Genotyping Array, 96 format

The Axiom OVSNP600 Genotyping Array, 96 format, contains 702,183 SNPs identified in free-ranging white-tailed deer and is a component of our Axiom Agrigenomics Genotyping solution where target genomic DNA is prepared and processed on the GeneTitan Multi-Channel instrument. The automated process include hybridization of the gDNA to this high-throughput microarray followed by staining, washing, imaging, and output of genotyping data for further analysis.

The Axiom OVSNP600 Genotyping Array was designed with input from the Michigan Department of Natural Resources, Iowa State University, University of Wisconsin-Milwaukee, Caesar Kleberg Wildlife Research Institute at Texas A&M University-Kingsville, and the USGS National Wildlife Health Center. The development of this array was supported by a grant from the Michigan Department of Natural Resources/Michigan State University Joint Wildlife Disease Initiative and a U.S. Fish and Wildlife Service Multi-State Conservation grant.

Use of this high-density array enables data generated in different laboratories to be more easily compared than data generated by other markers (e.g., microsatellites), facilitating collaboration among labs.

The Axiom OVSNP60 Genotyping Array is also available in 384HT format (Cat. No. 551367), containing probe sets for 72,723 SNPs.

Array content
• Probe sets for 702,183 SNPs identified in free-ranging white-tailed deer, selected from 89 million SNPs identified through whole-genome resequencing of free-ranging deer from Iowa, North Carolina, and Texas, and also selected to ensure even distribution across scaffolds of the reference genome (PRJNA317745)
• Includes 517,261 SNPs that fell into the “Best and Recommended” category in an initial screening of 480 free-ranging white-tailed deer collected from 15 states in the eastern range of the species

Applications
• Identify genomic regions under selection and identify candidate genes associated with disease susceptibility, local adaptation, or other phenotypes of interest
• Assign parentage and resolve relationships among individuals
• Assign individuals to population of origin and quantify hybridization
• Distinguish captive deer from free-ranging deer
• Conduct landscape genomics studies to identify landscape features that facilitate or limit deer movement/population connectivity

Required products
GeneChip Command Console Software
GeneTitan Multi-Channel Instrument
Axiom Analysis Suite Software
Axiom Long Format Export Tool (AxLE)