B-PER™ Reagent (in Phosphate Buffer)

B-PER II Reagent contains more detergent making it ideal for extracting proteins from small bacterial cultures with less than 20 mL in volume.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

B-PER Reagent utilizes a proprietary, mild nonionic detergent in a 20 mM Tris HCl, pH 7.5 buffer. No enzymatic components are present in the B-PER solution itself (B-PER Complete Bacterial Protein Extraction Reagent, Cat. No. 89821, 89822 does contain enzymes). Depending on your particular protein, you may need to add components such as salt, lysozyme, protease inhibitors, reducing agents and chelating agents. Other ready-to-use formats include B-PER II Bacterial Protein Extraction Reagent (2X), B-PER Reagent (in Phosphate Buffer), and B-PER with Enzymes Bacterial Protein Extraction Kit.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

For 1 gram of cells add 4 mL of B-PER Reagent.

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While this is protein-dependent, many proteins have been successfully tested in downstream applications (reporter assays, immunoprecipitation and beta-Gal assays), including GST and 6xHis proteins. Otherwise, samples can be diluted or dialyzed to remove any interfering substances.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

This is often an indication of the presence of large amounts of DNA in the extract. This viscosity will be greatly decreased upon addition of DNase I (Cat. No. 90083).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.