Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Citations & References (7)
Thermo Scientific™

Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X)は、便利な1つの溶液で細胞および組織ライセートのタンパク質サンプルを完全に保護します詳細を見る
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製品番号（カタログ番号）数量
7844610 mL
7844224 x 100 μL
784401 mL
784445 x 1 mL
製品番号（カタログ番号） 78446
価格（JPY）
233,800
Each
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数量:
10 mL
一括またはカスタム形式をリクエストする
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X)は、便利な1つの溶液で細胞および組織ライセートのタンパク質サンプルを完全に保護します。

Halt Protease and Phosphatase Inhibitor Cocktail (100X)の特長：

•完全な保護—オールインワンカクテルはプロテアーゼとホスファターゼの両阻害剤を含有
• 複数のパッケージサイズ—4つの便利なパッケージサイズから選択できるため、ほぼすべての実験規模に適合
• 便利—100倍濃縮組成は、個別の阻害剤やその他のフォ＝マットよりも使いやすく効果的
• 保存が容易—水性フォーマットはDMSOベースのカクテルとは異なり冷凍する必要がなく、低温室で保存可能
• 適合性—ピークシフトを引き起こす可能性のあるAEBSFを含んでいないため、質量分析（MS）に使用可能
• 検証済み—Thermo Scientific Pierce Cell Lysis Bufferに完全に適合に完全に適合

Halt Protease and Phosphatase Inhibitor Cocktailは、タンパク質の抽出や精製中にタンパク質が分解されるのを防ぐよう、特別に最適化されています。カクテルには、アミノペプチダーゼ、システインおよびセリンプロテアーゼ、セリン／スレオニンおよびタンパク質チロシンホスファターゼを標的とする主要分類のプロテアーゼおよびホスファターゼに対する阻害剤が含まれます。メタロプロテアーゼの阻害を促進するために、0.5 M EDTAが別のチューブで提供されます。

関連製品
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
研究用途にのみご使用ください。診断目的には使用できません。
仕様
バッファーLysis Buffer
阻害剤プロテアーゼ阻害剤、ホスファターゼ阻害剤
数量10 mL
試薬タイププロテアーゼ阻害剤、ホスファターゼ阻害剤
形状液体
製品ラインHalt
製品タイプProtease and Phosphatase Inhibitor Cocktail
Unit SizeEach
組成および保存条件
受け取り後、4℃で保存

よくあるご質問（FAQ）

Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?

The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?

Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?

Yes. Halt Protease, Halt Phosphatase and Protease/Phosphatase combined Inhibitor Cocktails are available. In addition, some of the individual inhibitors of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?

EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?

The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Halt™ Protease and Phosphatase Inhibitor Cocktail (100X), 10 mL FAQs

引用および参考文献 (7)

引用および参考文献
Abstract
A guide to selecting high-performing antibodies for human Midkine for use in Western blot and immunoprecipitation.
Authors:Ayoubi R,Southern K,Laflamme C,NeuroSGC/YCharOS Collaborative Group
Journal:F1000Research
PubMed ID:39092005
Midkine is a secreted protein that acts as a growth factor or cytokine involved in cell survival and inflammatory processes. It accumulates in amyloid plaques, which are hallmarks of Alzheimer's Disease (AD). The reproducibility of Midkine research would be enhanced if the community had access to well-characterized anti-Midkine antibodies. In ... More
Chemoproteomic discovery of a covalent allosteric inhibitor of WRN helicase.
Authors:Baltgalvis KA,Lamb KN,Symons KT,Wu CC,Hoffman MA,Snead AN,Song X,Glaza T,Kikuchi S,Green JC,Rogness DC,Lam B,Rodriguez-Aguirre ME,Woody DR,Eissler CL,Rodiles S,Negron SM,Bernard SM,Tran E,Pollock J,Tabatabaei A,Contreras V,Williams HN,Pastuszka MK,Sigler JJ,Pettazzoni P,Rudolph MG,Classen M,Brugger D,Claiborne C,Plancher JM,Cuartas I,Seoane J,Burgess LE,Abraham RT,Weinstein DS,Simon GM,Patricelli MP,Kinsella TM
Journal:Nature
PubMed ID:38658751
WRN helicase is a promising target for treatment of cancers with microsatellite instability (MSI) due to its essential role in resolving deleterious non-canonical DNA structures that accumulate in cells with faulty mismatch repair mechanisms(1-5). Currently there are no approved drugs directly targeting human DNA or RNA helicases, in part owing ... More
Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists.
Authors:Wang T,Chen X,Yang G,Shi X
Journal:Biomolecules
PubMed ID:38672473
There is a high demand for agonist biomolecules such as cytokine surrogates in both biological and medicinal research fields. These are typically sourced through natural ligand engineering or affinity-based screening, followed by individual functional validation. However, efficient screening methods for identifying rare hits within immense libraries are very limited. In ... More
hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels.
Authors:Gibson A, Lewis AP, Affleck K, Aitken AJ, Meldrum E, Thompson N
Journal:J Biol Chem
PubMed ID:15919655
'Proteins of the CLCA gene family have been proposed to mediate calcium-activated chloride currents. In this study, we used detailed bioinformatics analysis and found that no transmembrane domains are predicted in hCLCA1 or mCLCA3 (Gob-5). Further analysis suggested that they are globular proteins containing domains that are likely to be ... More
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Authors:Ståhlberg A, Thomsen C, Ruff D, Åman P
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. ... More
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