Triton™ X-100 Surfact-Amps™ Detergent Solution

One thing you should do is to permeabilize well, by including 0.1% to 0.2% Triton X-100 in all washes and incubations. Another recommendation is to extend the labeling times to overnight at 4^oC for both primary antibody and secondary antibody. Finally, label the section free-floating, rather than adhered to a slide, to gain penetration for the antibody from both sides.

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After opening, we recommend storing the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. Frequent opening should be avoided because Triton X-100 is susceptible to autoxidation upon exposure to air.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Triton X-100 Surfact-Amps Detergent Solution is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (20-25 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Thermo Scientific Pierce Surfact-Amps detergents are highly purified, precisely diluted (10%) formulations that are ideal for applications or assays that are sensitive to contaminants that are present in unpurified detergents. We test every batch to insure that our detergents contain less than 1.0 µeq/mL peroxides and carbonyls and package them under nitrogen, to prevent oxidization during storage.

Superior quality - lower measurable contaminant levels than other leading vendors
Accurate -precise 10% detergent solution in ultrapure water
Easy-to-use - solution is simple to dispense and dilute for use
Exceptionally pure - less than 1.0 µeq/mL peroxides and carbonyls

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Ionic detergents, or those that carry a charge, are the most likely to be denaturing to proteins. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein-protein interactions through changes in the three-dimensional structure of the proteins. Nondenaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS).



Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.