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Mitochondria Isolation Kit for Cultured Cells
Mitochondria Isolation Kit for Cultured Cells
Citations & References (8)
Thermo Scientific™

Mitochondria Isolation Kit for Cultured Cells

培養細胞用Thermo Scientificミトコンドリア単離キットは、約40分で、哺乳動物培養細胞からミトコンドリアをインタクトな状態で単離するためのマイクロ遠心分離チューブ法を提供します。培養細胞用ミトコンドリア単離キットの特長：•迅速 — 200万個の細胞から約40分でインタクトなミトコンドリアを単離（細胞収穫後）• フレキシブル詳細を見る
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製品番号（カタログ番号）数量
89874115 mL
製品番号（カタログ番号） 89874
価格（JPY）
67,900
Each
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数量:
115 mL
一括またはカスタム形式をリクエストする
培養細胞用Thermo Scientificミトコンドリア単離キットは、約40分で、哺乳動物培養細胞からミトコンドリアをインタクトな状態で単離するためのマイクロ遠心分離チューブ法を提供します。

培養細胞用ミトコンドリア単離キットの特長：

•迅速 — 200万個の細胞から約40分でインタクトなミトコンドリアを単離（細胞収穫後）
• フレキシブル — 試薬ベースの手法を使って多検体サンプルを同時に調整、またはダウンスホモジナイザー手法で最高収量を達成
• 最適化可能 — 試薬ベース手法での収量に対する純度の最適化のためのガイドラインを提供
• 簡単なベンチトップ型 — 分離はマイクロ遠心分離チューブで実行
• 適合性 — 単離されたミトコンドリアは界面活性剤で溶解、または二次元電気泳動を含むほとんどすべての下流アプリケーションで使用可能

一般的に、ミトコンドリアの単離は、ダウンスホモジナイザーを用いた単一試料処理を必要とします。Mitochondria Isolation Kitは、機械を使用しない試薬ベースの手法を採用しており、約40分で多検体（6）サンプルの調整が可能です。哺乳動物培養細胞ペレットは、独自の製剤を使って穏やかに溶解され、ミトコンドリアの完全性の損失を最小限に抑えながら最大限に回収します。

製品説明書で、収量パラメーターに対する純度パラメーターの最適化に関するガイドラインが示されています。本キットはまた、試薬ベース手法と比較して2倍のミトコンドリアを回収できる、最適化されたダウンス型ホモジナイザー手順も提供します。この2つの手順は、ベンチトップ型マイクロ遠心分離機でミトコンドリアおよび細胞質分画を分離する分画遠心法を採用しており、約40分で完了します（細胞収穫後）。単離されたミトコンドリアは、ミトコンドリアのプロテオミクス研究だけでなく、アポトーシス、シグナル伝達、代謝研究などの下流アプリケーションにも使用できます。

関連製品
Mitochondria Isolation Kit for Tissue
Lysosome Enrichment Kit for Tissues and Cultured Cells
研究用途にのみご使用ください。診断目的には使用できません。
仕様
数量115 mL
試薬タイプオルガネラ単離、細胞内分画
対応可能対象50単離
形状キット、液体
製品タイプMitochondria Isolation Kit
Unit SizeEach
組成および保存条件
納品後、4℃でキットを保管してください。製品は室温で出荷されます。

よくあるご質問（FAQ）

Can you provide the shelf-life for the Mitochondria Isolation Kit for Cultured Cells?

The Mitochondria Isolation Kit for Cultured Cells is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is a typical yield from the Mitochondria Isolation Kit for Cultured Cells?

The typical yield is approximately 150 µg protein from 20 million starting cells after mitochondrial isolation and lysis, based on HeLa cells. This varies with cell type.

Can I use a Dounce homogenization procedure in conjunction with the Mitochondria Isolation Kit for Cultured Cells (Cat. No. 89874)?

Yes, the kit offers an optimized Dounce homogenization procedure, which results in two-fold greater mitochondri recovery compared to reagent-based method.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I further purify my crude mitochondrial fraction isolated using your Mitochondria Isolation Kit for Cultured Cells (Cat. No. 89874)?

To obtain a more purified fraction of heavy mitochondria, centrifuge the post-nuclear supernatant at 3000g rather than 12,000g. Western blot analysis of purified versus crude mitochondrial fractions prepared with this kit result in a > 50% reduction in cathepsin S (lysosomal protein) and PMP70 (peroxisomal protein) in the purified fraction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What kits do you offer for cell fractionation?

We offer the following kits for cell fractionation:

Mem-PER Plus Membrane Protein Extraction Kit, Cat. No. 89842
NE-PER Nuclear and Cytoplasmic Extraction Reagents, Cat. No. 78835
Subcellular Protein Fractionation Kit for Cultured Cells, Cat. No. 78840
Subcellular Protein Fractionation Kit for Tissues, Cat. No. 87790
Mitochondrial Isolation Kit for Cultured Cells, Cat. No. 89874
Mitochondrial Isolation kit for Tissue, Cat. No. 89801
Lysosome Enrichment Kit for Tissues and Cultured Cells, Cat. No. 89839

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

View all Mitochondria Isolation Kit for Cultured Cells FAQs

引用および参考文献 (8)

引用および参考文献
Abstract
Hypoxic Regulation of Mitochondrial Metabolism and Mitophagy in Nucleus Pulposus Cells Is Dependent on HIF-1α-BNIP3 Axis.
Authors:Madhu V,Boneski PK,Silagi E,Qiu Y,Kurland I,Guntur AR,Shapiro IM,Risbud MV
Journal:Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
PubMed ID:32251541
Nucleus pulposus (NP) cells reside in an avascular and hypoxic microenvironment of the intervertebral disc and are predominantly glycolytic due to robust HIF-1 activity. It is generally thought that NP cells contain few functional mitochondria compared with cells that rely on oxidative metabolism. Consequently, the contribution of mitochondria to NP ... More
Hydrogen Peroxide Promotes the Production of Radiation-Derived EVs Containing Mitochondrial Proteins.
Authors:Miller CE,Xu F,Zhao Y,Luo W,Zhong W,Meyer K,Jayswal R,Weiss HL,St Clair WH,St Clair DK,Chaiswing L
Journal:Antioxidants (Basel, Switzerland)
PubMed ID:36358489
In spite of extensive successes, cancer recurrence after radiation treatment (RT) remains one of the significant challenges in the cure of localized prostate cancer (PCa). This study focuses on elucidating a novel adaptive response to RT that could contribute to cancer recurrence. Here, we used PC3 cell line, an adenocarcinoma ... More
Hypoxic Regulation of Mitochondrial Metabolism and Mitophagy in Nucleus Pulposus Cells Is Dependent on HIF-1α-BNIP3 Axis
Authors:Madhu V, Boneski PK, Silagi E, Qiu Y, Kurland I, Guntur AR, Shapiro IM, Risbud MV.
Journal:J Bone Miner Res
PubMed ID:32251541
Nucleus pulposus (NP) cells reside in an avascular and hypoxic microenvironment of the intervertebral disc and are predominantly glycolytic due to robust HIF-1 activity. It is generally thought that NP cells contain few functional mitochondria compared with cells that rely on oxidative metabolism. Consequently, the contribution of mitochondria to NP ... More
Direct administration of mesenchymal stem cell-derived mitochondria improves cardiac function after infarction via ameliorating endothelial senescence.
Authors:Liang X,Zhang Y,Lin F,Li M,Li X,Chen Y,Liu J,Meng Q,Ma X,Wang E,Wei L,He Z,Fan H,Zhou X,Ding Y,Liu Z
Journal:Bioengineering & translational medicine
PubMed ID:36684073
Mitochondrial dysfunction is considered to be a key contributor to the development of heart failure. Replacing injured mitochondria with healthy mitochondria to restore mitochondrial bioenergy in myocardium holds great promise for cardioprotection after infarction. This study aimed to investigate whether direct transplantation of exogenous mitochondria derived from mesenchymal stem cells ... More
Mitochondrial Transplantation Attenuates Cerebral Ischemia-Reperfusion Injury: Possible Involvement of Mitochondrial Component Separation.
Authors:Xie Q,Zeng J,Zheng Y,Li T,Ren J,Chen K,Zhang Q,Xie R,Xu F,Zhu J
Journal:Oxidative medicine and cellular longevity
PubMed ID:34849186
BACKGROUND: Mitochondrial dysfunctions play a pivotal role in cerebral ischemia-reperfusion (I/R) injury. Although mitochondrial transplantation has been recently explored for the treatment of cerebral I/R injury, the underlying mechanisms and fate of transplanted mitochondria are still poorly understood. METHODS: Mitochondrial morphology and function were assessed by fluorescent staining, electron microscopy, ... More
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