Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix
DISCONTINUATION NOTICE: Geltrex™ Reduced Growth Factor Matrices won't be restocked — switch to NEW Geltrex™ Flex Reduced Growth Factor Matrices with equivalent performance, expanded vial sizes, and an added organoid-qualified option.
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix
Citations & References (14)
Gibco™

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix

Geltrex™ LDEVフリー還元増殖因子基底膜マトリックスは、マウスEngelbreth-Holm-Swarm（EHS）腫瘍から抽出された基底膜の可溶性形態で、ラクトース脱水素酵素上昇ウイルス（LDEV）などのウイルスが含まれていません。Geltrex™マトリックスの主な構成要素は、ラミニン詳細を見る
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製品番号（カタログ番号）数量
A14132025 mL
A14132011 mL
NOTICE: This product will no longer be restocked — switch now to the replacement product Geltrex™ Flex Reduced Growth Factor matrix, delivering the same trusted performance in expanded vial sizes, along with a new organoid qualified matrix choice.
製品番号（カタログ番号） A1413202
現在の製品を選択する
Geltrex™ LDEVフリー還元増殖因子基底膜マトリックスは、マウスEngelbreth-Holm-Swarm（EHS）腫瘍から抽出された基底膜の可溶性形態で、ラクトース脱水素酵素上昇ウイルス（LDEV）などのウイルスが含まれていません。Geltrex™マトリックスの主な構成要素は、ラミニン、コラーゲンIV、エンタクチン、ヘパリン硫酸塩プロテオグリカンです。初代上皮細胞、内皮細胞、平滑筋細胞、ヒト人工多能性幹細胞（iPSC）など、さまざまな細胞培養における分化した表現型の促進および維持に最適です。Geltrex™マトリックスは、いくつかの増殖因子を平均2倍低減します。その結果、基底膜抽出物を必要とするアプリケーションにおける特異的な増殖因子の影響を、コントロールされた再現性のあるフォーマットで分析できます。

Geltrex™ LDEVフリー還元増殖因子基底膜マトリックスの主な特長：

• ラクトース脱水素酵素上昇ウイルス（LDEV）がないため、あらゆる種類の細胞培養およびマウスのin vivoアプリケーションに最適です
• ロット間で一貫したタンパク質濃度により、ロットのスクリーニングの必要性を低減できます
•、チューブ形成アッセイを含むさまざまなアプリケーション用のプロトコルが利用可能です

hESC研究での信頼性と一貫性を確保します
EHS腫瘍細胞処理における最近の進歩により、ウイルスフリーの基底膜抽出物が得られました。すべてののロットがRT-PCR法で試験されるため、Geltrex™のすべてのロットがウイルスフリーであると確信できます。さらに、当社の一貫した製造プロセスにより、ロット間のばらつきがほとんどなく、平均15 mg/mlのタンパク質濃度が保証されるため、すべてを安心してご購入いただけます。

検証済みGibco™プロトコルで血管新生を調べます
Geltrex™ LDEVフリー還元増殖因子基底膜マトリックスには多くのアプリケーションがあり、それぞれ異なる厚さと濃度が必要です。一般的に、初代細胞の分化研究では、>5 mg/mlのタンパク質濃度が使用されます。Geltrex™ LDEVフリー還元増殖因子基底膜マトリックスは、Gibco™初代細胞および特殊培地を用いたチューブ形成アッセイプロトコルの開発に使用されています。このプロトコルおよびその他の血管新生アッセイプロトコルをご覧ください
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞タイプVariety of cultured cells, including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC)
濃度12 to 18 mg/mL
エンドトキシンレベルLow
フォーマットTube(s)
製品ラインGibco
製品タイプBasement Membrane Matrix
純度または品質グレードCell Culture Grade
数量5 mL
品質保持期間36 months
出荷条件Dry Ice
種Mouse
分類Reduced Growth Factor
Culture TypeAdherent Cell Culture
形状Frozen
Serum LevelSerum-free
無菌性Sterile
添加剤なしNo Phenol Red
Unit SizeEach
組成および保存条件
Store at -20°C.

よくあるご質問（FAQ）

What growth factors are reduced in the Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413202)?

Although the standard basement membrane extract (BME) contains growth factors such as EGF, IGF‐1, PDGF and TGF‐beta, the reduced growth factor basement membrane extract product (Geltrex matrix) contains below detectable levels of EGF, PDGF and NGF. It still has some TGF‐beta (about 2 ng/mL) and 3 times less IGF‐1 on average than regular BME, hence the designation "reduced". Neither standard nor reduced BME contain NGF or FGF‐2.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center

What volume do you recommend for thin coating 35 mm dishes with Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413201, A1413202) or Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302)?

We recommend thin (non-gelling) coating 35 mm dishes with 1.5 mL of Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413201, A1413202) or Geltrex LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301, A1413302) prediluted 1/100 in DMEM/F-12 medium (1% final concentration).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I refreeze and reuse Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix after having used it in an experimental setup?

Yes, you can refreeze and reuse Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix. However, you should avoid multiple freeze/thaw cycles. hence, we would recommend the following:

  1. Thaw Geltrex Matrix in a refrigerator overnight at 2-8 degrees C.
  2. Mix Geltrex Matrix solution by slowly pipetting up and down. Be careful not to introduce air bubbles.
  3. If not using up the complete solution, prepare multiple aliquots, either of the original solution or the diluted one, and freeze.


Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I characterize human embryonic stem ( ES) cells?

Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).

How are human embryonic stem (ES) cells derived?

Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).

View all Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix, 5 mL FAQs

引用および参考文献 (14)

引用および参考文献
Abstract
Microgels on-demand.
Authors:Eydelnant IA, Betty Li B, Wheeler AR
Journal:
PubMed ID:24566526
'Three-dimensional (3D) hydrogel structures are finding use in fundamental studies of self-assembly, rheology, and 3D cell culture. Most techniques for 3D hydrogel formation are ''single pot'', in which gels are not addressable after formation. For many applications, it would be useful to be able to form arrays of gels bearing ... More
Lumen Formation Is an Intrinsic Property of Isolated Human Pluripotent Stem Cells.
Authors:Taniguchi K, Shao Y, Townshend RF, Tsai YH, DeLong CJ, Lopez SA, Gayen S, Freddo AM, Chue DJ, Thomas DJ, Spence JR, Margolis B, Kalantry S, Fu J, O'Shea KS, Gumucio DL
Journal:
PubMed ID:26626176
'We demonstrate that dissociated human pluripotent stem cells (PSCs) are intrinsically programmed to form lumens. PSCs form two-cell cysts with a shared apical domain within 20 hr of plating; these cysts collapse to form monolayers after 5 days. Expression of pluripotency markers is maintained throughout this time. In two-cell cysts, ... More
Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.
Authors:Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman MJ, Kopetz S, Ellis LM, Meng QH, Li S
Journal:
PubMed ID:25516888
This study aimed to detect cell-surface vimentin (CSV) on the surface of epithelial-mesenchymal transitioned (EMT) circulating tumor cells (CTC) from blood of patients with epithelial cancers. ... More
Induced pluripotent stem cell-derived mesenchymal stem cell seeding on biofunctionalized calcium phosphate cements.
Authors:TheinHan W, Liu J, Tang M, Chen W, Cheng L, Xu HH
Journal:
PubMed ID:24839581
Induced pluripotent stem cells (iPSCs) have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells (iPSC-MSCs), and investigate iPSC-MSC proliferati on and osteogenic differentiation on calcium phosphate cement (CPC) containing biofunctional agents for the first time. Human iPSCs ... More
Neural Differentiation of Spheroids Derived from Human Induced Pluripotent Stem Cells-Mesenchymal Stem Cells Coculture.
Authors:Song L, Tsai AC, Yuan X, Bejoy J, Sart S, Ma T, Li Y
Journal:Tissue Eng Part A
PubMed ID:29160172
'Organoids, the condensed three-dimensional (3D) tissues emerged at the early stage of organogenesis, are a promising approach to regenerate functional and vascularized organ mimics. While incorporation of heterotypic cell types, such as human mesenchymal stem cells (hMSCs) and human induced pluripotent stem cells (hiPSCs)-derived neural progenitors aid neural organ development, ... More
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