FluoroBrite™ DMEM

Citations & References (21)

Gibco™

FluoroBrite™ DMEM

Name: FluoroBrite™ DMEM
SKU: A1896702

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表示を変更

製品番号（カタログ番号）	数量
A1896702	10 x 500 mL
A1896701	500 mL

2 オプション

製品番号（カタログ番号） A1896702

価格（JPY）

45,800

Each

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数量:

10 x 500 mL

Gibco™ FluoroBrite™ DMEMは、バックグラウンド蛍光がPBSと同等であり、標準的なフェノールレッドフリーDMEMよりもバックグラウンド蛍光を90%低下させます。FluoroBrite™ DMEMは、ルーチン細胞培養に10%のウシ胎児血清と4mMのL-グルタミン酸、またはGlutaMAX™サプリメントを添加する場合を想定して、必要な栄養素が最初から含まれており、フルオロフォアのシグナル／ノイズ比が向上するように設計されています。そのため、最適な細胞の健康を促進する環境で微弱な蛍光事象も可視化できます。その他にも次のような特長があります。

•生細胞イメージング中の蛍光シグナルの改良
• 細胞の健康を維持するために役立つDMEMベース

生細胞蛍光顕微鏡は、根本的に重要で生理学的に関連する生物学的事象を視覚化するために不可欠な技術です。この手法の主要な課題の1つに、弱いフルオロフォアをイメージングする際に必ず細胞の損傷、光退色、または望ましくない細胞の健康変化が伴うことがあります。FluoroBrite™ DMEMは、これらの問題に対処するのに役立ちます。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

濃度1 X

製造品質cGMP-compliant under the ISO 13485 standard

製品タイプDMEM (Dulbecco's Modified Eagle Medium)

数量10 x 500 mL

品質保持期間12 Months From Date of Manufacture

分類Animal Origin-free

形状Liquid

Serum LevelStandard Serum Supplementation

無菌性Sterile-filtered

添加剤ありHigh Glucose

添加剤なしNo Glutamine, No Phenol Red, No HEPES, No Sodium Pyruvate

Unit SizeEach

組成および保存条件

Store in refrigerator (2–8°C). Protect from light.

よくあるご質問（FAQ）

Do you offer an alternative or replacement of Invitrogen Live Cell Imaging Solution (Cat. No. A14291DJ)?

An alternative for Live Cell Imaging Solution (LCIS) (Cat. No. A14291DJ) is FluoroBrite DMEM (Cat. Nos. A1896701, A1896702), which, like LCIS, is formulated not to have any fluorescent background or quenchers and is verified for imaging live cells in fluorescence. The product can be found using the link below.

FluoroBrite DMEM (Cat. Nos. A1896701, A1896702)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the osmolality of Fluorobrite DMEM?

We do provide osmolality information on the certificate of analysis. All lots of Fluorobrite DMEM (Cat. Nos. A1896701 and A1896702) will meet the osmolality specification of 320-350 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (21)

引用および参考文献

Abstract

Open source software for quantification of cell migration, protrusions, and fluorescence intensities.

Authors:Barry DJ, Durkin CH, Abella JV, Way M,

Journal:

PubMed ID:25847537

'Cell migration is frequently accompanied by changes in cell morphology (morphodynamics) on a range of spatial and temporal scales. Despite recent advances in imaging techniques, the application of unbiased computational image analysis methods for morphodynamic quantification is rare. For example, manual analysis using kymographs is still commonplace, often caused by ... More

A BRCA1-interacting lncRNA regulates homologous recombination.

Authors:Sharma V, Khurana S, Kubben N, Abdelmohsen K, Oberdoerffer P, Gorospe M, Misteli T,

Journal:

PubMed ID:26412854

Long non-coding RNAs (lncRNAs) are important players in diverse biological processes. Upon DNA damage, cells activate a complex signaling cascade referred to as the DNA damage response (DDR). Using a microarray screen, we identify here a novel lncRNA, DDSR1 (DNA damage-sensitive RNA1), which is induced upon DNA damage. DDSR1 induction ... More

Ebola Virus and Severe Acute Respiratory Syndrome Coronavirus Display Late Cell Entry Kinetics: Evidence that Transport to NPC1+ Endolysosomes Is a Rate-Defining Step.

Authors:Mingo RM, Simmons JA, Shoemaker CJ, Nelson EA, Schornberg KL, D'Souza RS, Casanova JE, White JM,

Journal:

PubMed ID:25552710

Ebola virus (EBOV) causes hemorrhagic fevers with high mortality rates. During cellular entry, the virus is internalized by macropinocytosis and trafficked through endosomes until fusion between the viral and an endosomal membrane is triggered, releasing the RNA genome into the cytoplasm. We found that while macropinocytotic uptake of filamentous EBOV ... More

3D imaging of Sox2 enhancer clusters in embryonic stem cells.

Authors:Liu Z, Legant WR, Chen BC, Li L, Grimm JB, Lavis LD, Betzig E, Tjian R,

Journal:

PubMed ID:25537195

Combinatorial cis-regulatory networks encoded in animal genomes represent the foundational gene expression mechanism for directing cell-fate commitment and maintenance of cell identity by transcription factors (TFs). However, the 3D spatial organization of cis-elements and how such sub-nuclear structures influence TF activity remain poorly understood. Here, we combine lattice light-sheet imaging, ... More

Microtubule-dependent transport and dynamics of vimentin intermediate filaments.

Authors:Hookway C, Ding L, Davidson MW, Rappoport JZ, Danuser G, Gelfand VI,

Journal:

PubMed ID:25717187

We studied two aspects of vimentin intermediate filament dynamics-transport of filaments and subunit exchange. We observed transport of long filaments in the periphery of cells using live-cell structured illumination microscopy. We studied filament transport elsewhere in cells using a photoconvertible-vimentin probe and total internal reflection microscopy. We found that filaments ... More

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