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Gibco™
SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red
SILAC Advanced DMEM/F-12 Flex is Advanced DMEM/F-12 without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| A2494301 | 500 mL |
製品番号（カタログ番号） A2494301
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18,800
Each
数量:
500 mL
SILAC Advanced DMEM/F-12 Flex is Advanced DMEM/F-12 without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotopic labeled lysine and/or arginine. Advanced DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells, which reduces the FBS requirements by 50-90% without any changes in growth rate or morphology. When supplemented with 1-2 % FBS, Advanced DMEM/F-12 Flex is capable of supporting cellular proliferation and maximum cell densities comparable to the conventional basal formulation supplemented with 5-10% FBS. Cells successfully cultured in Advanced DMEM/F-12 with no adaptation include Jurkat, WI-38, SP2, Vero, and MRC-5. We offer a variety of DMEM/F-12 modifications for a range of applications. Find the right formulation using the media selector tool.
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 typically requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement (optional). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.
cGMP Manufacturing and Quality System
SILAC Advanced DMEM/F-12 Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
This Advanced DMEM/F-12 is manufactured as follows:
| With | Without |
| • Non-essential amino acids | • Glucose |
| • Sodium pyruvate | • Phenol red |
| • L-arginine | |
| • L-glutamine | |
| • L-lysine | |
| • HEPES | |
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 typically requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement (optional). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.
cGMP Manufacturing and Quality System
SILAC Advanced DMEM/F-12 Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
研究または製造用途です。診断目的には使用できません。
仕様
細胞タイプMammalian Cells
製品ラインGibco
製品タイプDulbecco's Modified Eagle Medium
数量500 mL
品質保持期間12 Months
Culture TypeMammalian Cell Culture
形状Liquid
Serum LevelReduced Serum
添加剤ありSodium Pyruvate, Non Essential Amino Acids (NEAA)
添加剤なしNo Glucose, No Glutamine, No HEPES, No Phenol Red
Unit SizeEach
組成および保存条件
Store in refrigerator (2–8°C) and protect from light.
Shelf life: 12 months from date of manufacture.
Shelf life: 12 months from date of manufacture.