Rapid transport of internalized P-selectin to late endosomes and the TGN: roles in regulating cell surface expression and recycling to secretory granules.
Authors:Straley KS, Green SA
Journal:J Cell Biol
PubMed ID:11018057
'Prior studies on receptor recycling through late endosomes and the TGN have suggested that such traffic may be largely limited to specialized proteins that reside in these organelles. We present evidence that efficient recycling along this pathway is functionally important for nonresident proteins. P-selectin, a transmembrane cell adhesion protein involved ... More
Targeting a neuropeptide to discrete regions of the motor arborizations of a single neuron.
Authors:Kueh D, Jellies JA,
Journal:J Exp Biol
PubMed ID:22623199
'The heart excitor (HE) motor neuron in the leech Hirudo releases acetylcholine (ACh) and a peptide, FMRFamide, to regulate the contractile activity of the heart tube and associated side vessels. Consistent with Dale''s principle, it was assumed that both neurotransmitters were localized to all presynaptic varicosities. However, we found discrete ... More
Intersectin-2L regulates caveola endocytosis secondary to Cdc42-mediated actin polymerization.
Authors:Klein IK, Predescu DN, Sharma T, Knezevic I, Malik AB, Predescu S,
Journal:J Biol Chem
PubMed ID:19622753
'Here we addressed the role of intersectin-2L (ITSN-2L), a guanine nucleotide exchange factor for the Rho GTPase Cdc42, in the mechanism of caveola endocytosis in endothelial cells (ECs). Immunoprecipitation and co-localization studies showed that ITSN-2L associates with members of the Cdc42-WASp-Arp2/3 actin polymerization pathway. Expression of Dbl homology-pleckstrin homology (DH-PH) ... More
Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
'The identification of alternative splice variants of Survivin that possess distinct functions from those originally identified for the main Survivin isoform has greatly increased the complexity of our understanding of the role of Survivin in different cells. Previous functional studies of the Survivin splice variants have been performed almost exclusively ... More
Improved immunocytochemical detection of biotinylated probes with Neutralite avidin.