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Citations & References (10)

Gibco™

StemFlex™ Medium

Gibco™ StemFlex™培地は、フィーダーフリー多能性幹細胞（PSC）の安定した増殖をサポートし、単細胞継代、遺伝子編集およびリプログラミングなどの新しいアプリケーションをサポートするように最適化されています。StemFlex 培地’は独自の組成により、柔軟なフィードスケジュール詳細を見る

製品番号（カタログ番号）	数量
A3349401	500 mL

製品番号（カタログ番号） A3349401

価格（JPY）

48,200

500 mL

Gibco™ StemFlex™培地は、フィーダーフリー多能性幹細胞（PSC）の安定した増殖をサポートし、単細胞継代、遺伝子編集およびリプログラミングなどの新しいアプリケーションをサポートするように最適化されています。StemFlex 培地’は独自の組成により、柔軟なフィードスケジュール（週末を含まないオプションを含む）が可能で、また、特定のアプリケーションに最適なマトリックスと継代試薬を選択することができます。StemFlex培地は、核型異常を伴わないPSCの長期的なフィーダーフリー培養を可能にし、3つの生殖細胞層すべてに分化する細胞の能力を維持します。

優れた結果
’StemFlex培地は、リプログラミング、単細胞培養、遺伝子編集など、PSC培養にストレスを与える今日のより困難なアプリケーションにおいて、優れた結果をもたらすように配合されています。StemFlex培地は、大手競合他社（mTeSR1）と比較して優れた細胞生存率を実現し、もっとも関連性の高い細胞種での成功を可能にします。

柔軟性
StemFlex培地は、これまでにない3つの方法でPSC培養条件に柔軟に対応することができます。週末フリーオプションを含む最適なフィードスケジュールの選択、ワークフローのニーズに応じたマトリックスの選択、必要な細胞解離のレベルに応じた継代試薬の選択などです。

卓越した価値と性能
StemFlex培地は、便利な2成分キット（450 mL基本培地と50 mL補液）で提供され、Geltrex™ LDEVフリー、hESC適格、抑制増殖因子基底膜マトリックスと併用することで、ヒトPSCの優れたフィーダーフリー培養のための費用対効果に優れた堅牢なシステムを提供します。週末を必要としないPSCの培養を可能にするStemFlex培地’の機能は、現在市場に出回っているフィーダーフリーのPSC培地の中でも、他に類を見ない価値を提供しています。

研究用途にのみご使用ください。診断目的には使用できません。

細胞株Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)

細胞タイプEmbryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)

濃度StemFlex Basal Medium (1X); StemFlex Supplement (10X)

製造品質cGMP for medical devices, 21 CFR Part 820 and ISO 13485

製品ラインStemFlex

製品タイプStem Cell Media

数量500 mL

品質保持期間24 months From Date of Manufacture

出荷条件Supplement ships on dry ice, basal can ship at RT

分類Animal Origin

Culture TypeAdherent Cell Culture, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)

形状Liquid

Serum LevelSerum-free

無菌性Sterile-filtered

Sterilization MethodSterile-filtered

添加剤ありPhenol Red

Unit SizeEach

組成および保存条件

• 450 mL Basal Medium, store at 2–8°C and protect from light
• 50 mL Supplement, store at -5 to -20°C and protect from light

よくあるご質問（FAQ）

What is the glucose content of the StemFlex Medium (Cat. No. A3349401)?

The StemFlex Medium (Cat. No. A3349401) includes 3.151 g/L (~17.5 mM) of D-glucose (dextrose) in the Basal Medium. The StemFlex Medium Supplement does not include glucose.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What media do you recommend for standard single-cell cloning of iPSCs?

We recommend StemFlex Medium (Cat. No. A3349401) or Essential 8 (Cat. No. A1517001) for standard single-cell iPSC cloning in the presence of RevitaCell or ROCK inhibitor. StemScale PSC Suspension Medium (Cat. No. A4965001) is not recommended for iPSC cloning.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does StemFlex Medium contain pen/strep antibiotic?

StemFlex Medium does not contain pen/strep antibiotic.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between StemFlex Medium and Essential 8 Flex Medium?

Essential 8 Flex Medium is a serum-free, xeno-free medium that contains proprietary material to extend the activity of the key heat-sensitive component, FGF2. Therefore, it supports the culture and expansion of feeder-free pluripotent stem cells (PSCs) without the need for daily or weekend feeding. Note: We also offer Essential 8 Medium that does not allow skipping of PSC feeding during the weekend.

StemFlex Medium also supports the expansion of feeder-free PSCs with a flexible feeding schedule (including weekend-free feeding) and also the ability to choose the matrix and passaging reagent that best suits specific applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Has StemFlex Medium been evaluated for growth of mouse ESCs?

StemFlex Medium contains basic FGF which has been shown to result in differentiation of mouse embryonic stem cells (mESCs) to mouse-derived epiblast stem cells (mEpiSCs). Also, mESCs demonstrate dependence on leukemia inhibitor factor (LIF). Therefore, StemFlex Medium is not recommended for culture of mESCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all StemFlex™ Medium FAQs

引用および参考文献 (10)

引用および参考文献

Abstract

Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media.

Authors:Daniszewski M, Nguyen Q, Chy HS, Singh V, Crombie DE, Kulkarni T, Liang HH, Sivakumaran P, Lidgerwood GE, Hernández D, Conquest A, Rooney LA, Chevalier S, Andersen SB, Senabouth A, Vickers JC, Mackey DA, Craig JE, Laslett AL, Hewitt AW, Powell JE, Pébay A

Journal:iScience

PubMed ID:30267684

'We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells ... More

Improving single-cell cloning workflow for gene editing in human pluripotent stem cells.

Authors:Chen YH, Pruett-Miller SM

Journal:Stem Cell Res

PubMed ID:30099335

'The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens. Unfortunately, the procedures for genome editing of hPSCs often subject cells to harsh conditions that compromise viability: a major problem that is compounded by ... More

Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing.

Authors:Weisheit I, Kroeger JA, Malik R, Klimmt J, Crusius D, Dannert A, Dichgans M, Paquet D

Journal:Cell Rep

PubMed ID:32460021

'CRISPR genome editing is a promising tool for translational research but can cause undesired editing outcomes, both on target at the edited locus and off target at other genomic loci. Here, we investigate the occurrence of deleterious on-target effects (OnTEs) in human stem cells after insertion of disease-related mutations by ... More

Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells.

Authors:Chen SW, Himeno M, Koui Y, Sugiyama M, Nishitsuji H, Mizokami M, Shimotohno K, Miyajima A, Kido T

Journal:Sci Rep

PubMed ID:32873852

'Hepatocytes derived from human iPSCs are useful to study hepatitis B virus (HBV) infection, however infection efficiency is rather poor. In order to improve the efficiency of HBV infection to iPSC-derived hepatocytes, we set a co-culture of hepatocytes with liver non-parenchymal cells and found that liver sinusoidal endothelial cells (LSECs) ... More

A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons.

Authors:Sridharan B, Hubbs C, Llamosas N, Kilinc M, Singhera FU, Willems E, Piper DR, Scampavia L, Rumbaugh G, Spicer TP

Journal:Sci Rep

PubMed ID:31227747

'Neurons created from human induced pluripotent stem cells (hiPSCs) provide the capability of identifying biological mechanisms that underlie brain disorders. IPSC-derived human neurons, or iNs, hold promise for advancing precision medicine through drug screening, though it remains unclear to what extent iNs can support early-stage drug discovery efforts in industrial-scale ... More

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