The Applied Biosystems QuantStudio Absolute Q MAP16 Plate Kit is a key component of the QuantStudio Absolute Q Digital PCR System. The system is powered by the patented microfluidic array plate (MAP) technology that enables consistent and automated separation of the sample into 20,000 microchambers with industry leading <1% coefficient of variation (CV). The QuantStudio Absolute Q MAP16 consumable uses microinjection-molded plates to overcome common challenges of inconsistency and high dead volume. This novel approach provides many benefits including: precise microchamber volume, consistent sample distribution, and greater than 98% of loaded volume analyzed.
For Research Use Only. Not for use in diagnostic procedures
仕様
使用対象(アプリケーション)Digital PCR
使用対象 (装置)QuantStudioTM Absolute QTM Digital PCR System
箱あたりの入数12 plates
パックあたり入数12 plates
数量12 Plates
出荷条件Room Temperature
バーコードIncludes Barcode
ウェル数16
Unit SizeEach
組成および保存条件
Includes MAP16 plates, gaskets, and Absolute Q Isolation Buffer (Cat. No. A52730)
よくあるご質問(FAQ)
I finished running my experiment on the QuantStudio Absolute Q Digital PCR instrument but just realized that I had selected the wrong dyes during setup. Can I change the dyes that were used after the run?
If dye channels are turned off before the run, then data will not be collected for them as the run progresses. This means that changing the dyes after the run will not be possible.
I ran my experiment using the QuantStudio Absolute Q Digital PCR System but did not actually use dyes in all the channels. Is there a way to only see those dye channels that were used?
Unused dye channels can be turned off after the run by going to the SETUP tab and clicking EDIT SETUP and then EDIT GROUPS. Here, the Analysis can be changed to Not Used. After clicking SAVE twice, the data will be reanalyzed and the unused dye channels will not be present in the analysis.
I'm using the QuantStudio Absolute Q Digital PCR System and the software is saying that the concentration is NaN. What does that mean?
NaN stands for not a number. The software will display NaN instead of an actual number when it has detected some issue during the analysis of the array images.
With the QuantStudio Absolute Q Digital PCR instrument, the run data didn't automatically transfer after the run completed. How can I get the data?
We would recommend restarting the software. If the run still doesn't transfer, we would recommend closing the software, rebooting the instrument, and then starting the software. If further assistance is needed, please contact Technical Support at techsupport@thermofisher.com.
With the QuantStudio Absolute Q Digital PCR System, how much sample can be loaded into each well?
9 µL of sample is loaded into each well along with 15 µL of isolation buffer. Loading more or less of either the sample or isolation buffer will affect filling of the microchambers and the run results.