Applied Biosystems™

Absolute Q™ DNA Digital PCR Master Mix (5X)

Catalog number: A52490
Applied Biosystems™

Absolute Q™ DNA Digital PCR Master Mix (5X)

Catalog number: A52490
Catalog Number
A52490
Unit Size
200 reactions
Price (USD)
286.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A52490200 reactions
286.00
Your Price:
***
Product Overview
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Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps. The 5X formulation enables analysis of higher sample volumes and delivers accurate quantification of DNA targets without using a standard curve.

Features of Absolute Q DNA Digital PCR Master Mix include:
Fast time-to-results—5 minutes of hands-on time and results in ~90 minutes
Simple workflow—set up the reaction like qPCR, then load and run the plate in a single instrument
High sensitivity—ability to detect down to 0.1% mutant allele frequency
Optimized for multiplexing—detect up to four targets in a single reaction

Simple and efficient workflow
The Absolute Q system simplifies the entire dPCR workflow. Prepare the dPCR reaction mix as you would for qPCR, pipette the reaction mix and isolation buffer into the microfluidic array plate (MAP), and place the plate in the QuantStudio Absolute Q Digital PCR System. Compartmentalization, thermal cycling, and data acquisition of up to four targets occur in a single instrument.

High sensitivity
Absolute Q DNA Digital PCR Master Mix (5X) enables analysis of >95% of the loaded reaction and consistently generates >20,000 microreactions, which maximizes sensitivity. Absolute Q DNA Digital PCR Master Mix (5X) can detect down to 0.1% mutant allele frequency when paired with Absolute Q liquid biopsy dPCR assays.

Maximum specificity and accuracy
Absolute Q DNA Digital PCR Master Mix (5X) is formulated with ROX dye for accurate micro-chamber analysis and with hot-start Taq DNA polymerase and dUTP/UDG to deliver high specificity and carryover contamination control.

Related products
Absolute Q Liquid Biopsy dPCR assays
Absolute Q Viral Titer dPCR assays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
5X
Detection Method
Primer-Probe Detection
Form
Liquid
No. of Reactions
200 Reactions
PCR Method
Digital PCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Fast
Sample Type
DNA, cDNA
Volume
360 µL
For Use With (Application)
Digital PCR (dPCR)
For Use With (Equipment)
QuantStudio Absolute Q Digital PCR System
Primer Probe Compatibility
TaqMan
Product Line
Absolute Q
Product Type
Digital PCR Master Mix
Shipping Condition
Dry Ice
Storage Requirements
Store at -30°C to -10°C
Sufficient For
200 Reactions

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