Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps. The 5X formulation enables analysis of higher sample volumes and delivers accurate quantification of DNA targets without using a standard curve.

Features of Absolute Q DNA Digital PCR Master Mix include:
• Fast time-to-results—5 minutes of hands-on time and results in ∼90 minutes
• Simple workflow—set up the reaction like qPCR, then load and run the plate in a single instrument
• High sensitivity—ability to detect down to 0.1% mutant allele frequency
• Optimized for multiplexing—detect up to four targets in a single reaction

Simple and efficient workflow
The Absolute Q system simplifies the entire dPCR workflow. Prepare the dPCR reaction mix as you would for qPCR, pipette the reaction mix and isolation buffer into the microfluidic array plate (MAP), and place the plate in the QuantStudio Absolute Q Digital PCR System. Compartmentalization, thermal cycling, and data acquisition of up to four targets occur in a single instrument.

High sensitivity
Absolute Q DNA Digital PCR Master Mix (5X) enables analysis of >95% of the loaded reaction and consistently generates >20,000 microreactions, which maximizes sensitivity. Absolute Q DNA Digital PCR Master Mix (5X) can detect down to 0.1% mutant allele frequency when paired with Absolute Q liquid biopsy dPCR assays.

Maximum specificity and accuracy
Absolute Q DNA Digital PCR Master Mix (5X) is formulated with ROX dye for accurate micro-chamber analysis and with hot-start Taq DNA polymerase and dUTP/UDG to deliver high specificity and carryover contamination control.

DNA methylation analysis
For DNA methylation analysis, use Absolute Q 1-step RT-dPCR Master Mix (4X), which does not contain UNG.

Related products
Absolute Q Liquid Biopsy dPCR assays
Absolute Q Viral Titer dPCR assays