BacLight™ Green Bacterial Stain

Citations & References (4)

Invitrogen™

BacLight™ Green Bacterial Stain

Bacライトグリーン（カタログ番号B-35000）および Bacライトレッド（カタログ番号B-35001）細菌染色は、細菌の検出およびモニタリング用の蛍光標識試薬です。これら2種類の色素は核酸染色剤ではありません。BacLight GreenおよびBacLight詳細を見る

製品番号（カタログ番号）	数量
B35000	20 x 50 μg

製品番号（カタログ番号） B35000

価格（JPY）

81,600

お問い合わせください ›

20 x 50 μg

Bacライトグリーン（カタログ番号B-35000）および Bacライトレッド（カタログ番号B-35001）細菌染色は、細菌の検出およびモニタリング用の蛍光標識試薬です。これら2種類の色素は核酸染色剤ではありません。BacLight GreenおよびBacLight Red細菌染色で染色された細菌は明るい緑色および赤色の蛍光（それぞれ吸収／発光が約480/516および約581/644 nm）を示します。これにより、適切なフローサイトメーターチャンネルまたは蛍光顕微鏡フィルターを使用して分解することが可能です。BacLight細菌染色剤は、ホルムアルデヒドまたはアルコール固定法に適合します。

微生物分析製品のすべてに関する追加情報をご覧ください。

研究用にのみ使用できます。診断用には使用いただけません。

細胞タイプ細菌

検出法蛍光

染色剤タイプその他の標識または色素

フォーマットチューブ、スライド

数量20 x 50 μg

出荷条件室温

溶解性DMSO（ジメチルスルホキシド）

Emission480⁄561

使用対象 (装置)蛍光顕微鏡, フローサイトメーター

製品ラインBacLight

製品タイプ細菌染色

Unit SizeEach

組成および保存条件

20バイアルのBacLight™グリーン細菌染色液（バイアル1本あたり50 ug）を含みます。フリーザー（-5～-30度）に保存し、遮光してください。

よくあるご質問（FAQ）

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What optical filter do your recommend with the BacLight Green Bacterial Stain?

We recommend filters suitable for fluorescein/FITC with the BacLight Green kit.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (4)

引用および参考文献

Abstract

Identification of broadly protective human antibodies to Pseudomonas aeruginosa exopolysaccharide Psl by phenotypic screening.

Authors:DiGiandomenico A, Warrener P, Hamilton M, Guillard S, Ravn P, Minter R, Camara MM, Venkatraman V, Macgill RS, Lin J, Wang Q, Keller AE, Bonnell JC, Tomich M, Jermutus L, McCarthy MP, Melnick DA, Suzich JA, Stover CK,

Journal:J Exp Med

PubMed ID:22734046

Pseudomonas aeruginosa is a leading cause of hospital-associated infections in the seriously ill, and the primary agent of chronic lung infections in cystic fibrosis patients. A major obstacle to effective control of P. aeruginosa infections is its intrinsic resistance to most antibiotic classes, which results from chromosomally encoded drug-efflux systems ... More

Analysis of adherence, biofilm formation and cytotoxicity suggests a greater virulence potential of Gardnerella vaginalis relative to other bacterial-vaginosis-associated anaerobes.

Authors:Patterson JL, Stull-Lane A, Girerd PH, Jefferson KK,

Journal:Microbiology

PubMed ID:19910411

'Worldwide, bacterial vaginosis (BV) is the most common vaginal disorder in women of childbearing age. BV is characterized by a dramatic shift in the vaginal microflora, involving a relative decrease in lactobacilli, and a proliferation of anaerobes. In most cases of BV, the predominant bacterial species found is Gardnerella vaginalis. ... More

Teleost skin, an ancient mucosal surface that elicits gut-like immune responses.

Authors:Xu Z, Parra D, Gómez D, Salinas I, Zhang YA, von Gersdorff Jørgensen L, Heinecke RD, Buchmann K, LaPatra S, Sunyer JO,

Journal:

PubMed ID:23884653

'Skin homeostasis is critical to preserve animal integrity. Although the skin of most vertebrates is known to contain a skin-associated lymphoid tissue (SALT), very little is known about skin B-cell responses as well as their evolutionary origins. Teleost fish represent the most ancient bony vertebrates containing a SALT. Due to ... More

SadA, a trimeric autotransporter from Salmonella enterica serovar Typhimurium, can promote biofilm formation and provides limited protection against infection.

Authors:Raghunathan D, Wells TJ, Morris FC, Shaw RK, Bobat S, Peters SE, Paterson GK, Jensen KT, Leyton DL, Blair JM, Browning DF, Pravin J, Flores-Langarica A, Hitchcock JR, Moraes CT, Piazza RM, Maskell DJ, Webber MA, May RC, MacLennan CA, Piddock LJ, Cunningham AF, Henderson IR,

Journal:Infect Immun

PubMed ID:21859856

Salmonella enterica is a major cause of morbidity worldwide and mortality in children and immunocompromised individuals in sub-Saharan Africa. Outer membrane proteins of Salmonella are of significance because they are at the interface between the pathogen and the host, they can contribute to adherence, colonization, and virulence, and they are ... More