BTC, AM, cell permeant

Citations & References (18)

Invitrogen™

BTC, AM, cell permeant

BTC、AMは、細胞透過性のクマリンベンゾチアゾール系Ca2+インジケーターです。Ca2+を結合すると、最大励起波長は約480 nmから400 nmにシフトします。これにより、不均一な色素ローディング細胞の厚さ、光退色詳細を見る

製品番号（カタログ番号）	数量
B6791	100 μg

製品番号（カタログ番号） B6791

価格（JPY）

56,800

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100 μg

BTC、AMは、細胞透過性のクマリンベンゾチアゾール系Ca²⁺インジケーターです。Ca²⁺を結合すると、最大励起波長は約480 nmから400 nmにシフトします。これにより、不均一な色素ローディング細胞の厚さ、光退色、および色素の漏出に本質的に依存しない、比測定が可能になります。BTCは、Ca²⁺およびK_d約7 μMに対して高い選択性を有しており、細胞内の高濃度のCa²⁺を定量できます^。

カルシウム、カリウム、pH、膜電位インジケータなどのイオンインジケーターの詳細についてご覧ください ›

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

数量100 μg

出荷条件室温

使用対象（アプリケーション）細胞の生存率と増殖

使用対象 (装置)フローサイトメーター

製品タイプ亜鉛インジケータ

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

引用および参考文献 (18)

引用および参考文献

Abstract

AM-loading of fluorescent Ca2+ indicators into intact single fibers of frog muscle.

Authors:Zhao M, Hollingworth S, Baylor SM

Journal:Biophys J

PubMed ID:9168048

'The AM loading of a number of different fluorescent Ca2+ indicators was compared in intact single fibers of frog muscle. Among the 13 indicators studied, loading rates (the average increase in the fiber concentration of indicator per first 60 min of loading) varied approximately 100-fold, from approximately 3 microM/h to ... More

Significance of Na/Ca exchange for Ca2+ buffering and electrical activity in mouse pancreatic beta-cells.

Authors:Gall D, Gromada J, Susa I, Rorsman P, Herchuelz A, Bokvist K

Journal:Biophys J

PubMed ID:10096898

'We have combined the patch-clamp technique with microfluorimetry of the cytoplasmic Ca2+ concentration ([Ca2+]i) to characterize Na/Ca exchange in mouse beta-cells and to determine its importance for [Ca2+]i buffering and shaping of glucose-induced electrical activity. The exchanger contributes to Ca2+ removal at [Ca2+]i above 1 microM, where it accounts for ... More

Photolysis-induced suppression of inhibition in rat hippocampal CA1 pyramidal neurons.

Authors:Wang J, Zucker RS

Journal:J Physiol

PubMed ID:11410632

'1. Whole cell patch clamp recording, Ca(2+) measurement with ratiometric fluorescent dyes and photolysis of caged Ca(2+) were combined to investigate the depolarization- and photolysis-induced suppression of inhibition (DSI and PSI) in rat hippocampal CA1 pyramidal cells. 2. A 5-s depolarization from -70 mV to 0 mV or a 6-s ... More

Ionized intracellular calcium concentration predicts excitotoxic neuronal death: observations with low-affinity fluorescent calcium indicators.

Authors:Hyrc K, Handran SD, Rothman SM, Goldberg MP

Journal:J Neurosci

PubMed ID:9254679

'Cytosolic calcium ([Ca2+]i) is an important mediator of neuronal signal transduction, participating in diverse biochemical reactions that elicit changes in synaptic efficacy, metabolic rate, and gene transcription. Excessive [Ca2+]i also has been implicated as a cause of acute neuronal injury, although measurement of [Ca2+]i in living neurons by fluorescent calcium ... More

Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC.

Authors:Hyrc KL, Bownik JM, Goldberg MP

Journal:Cell Calcium

PubMed ID:10756974

'Accurate measurement of elevated intracellular calcium levels requires indicators with low calcium affinity and high selectivity. We examined fluorescence spectral properties and ionic specificity of three low-affinity, ratiometric indicators structurally related to Fura-2: mag-Fura-2 (furaptra), Fura-2FF, and BTC. The indicators differed in respect to their excitation wavelengths, affinity for Ca2+ ... More

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