CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Citations & References (7)

Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Name: CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit
SKU: C10740

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表示を変更

製品番号（カタログ番号）	数量
C10740	20アッセイ
C10427	100アッセイ

2 オプション

製品番号（カタログ番号） C10740

価格（JPY）

20,700

Each

お問い合わせください ›

数量:

20アッセイ

CellEvent™カスパーゼ-3/7 Greenフローサイトメトリーアッセイキットは、アポトーシスを起こしている細胞における活性化されたカスパーゼ-3およびカスパーゼ-7のフローサイトメトリー検出を可能にします。このキットは、新しい蛍光発生基質CellEvent™カスパーゼ-3/7 Green検出試薬およびSYTOX™ AADvanced™死細胞染色剤を含みます。

•Caspase-3/7には、活性化されたカスパーゼ-3およびカスパーゼ-7のための認識配列が含まれます
• 識別が容易—生細胞、死細胞、およびアポトーシスを起こしている細胞の集団を明確に識別します
• 迅速な分析—洗浄と固定は不要です
• マルチカラー対応—488 nmレーザーまたは他のレーザーで励起可能な他の色素と結合します

CellEvent™カスパーゼ-3/7 Green検出試薬は、核酸結合色素に結合した4アミノ酸ペプチド（DEVD）で構成される細胞透過性試薬です。アポトーシス中、カスパーゼ-3およびカスパーゼ-7タンパク質が活性化され、DEVDペプチドにエンコードされたカスパーゼ3/7認識配列を開裂できます。認識配列の開裂および試薬によるDNA結合は、アポトーシスを起こしている細胞に約511/533 nmの最大吸収／最大発光を持つ明るい蛍光シグナルを標識します。SYTOX™ AADvanced™死細胞染色剤と併用すると、アポトーシスを起こしている細胞を生細胞および壊死細胞から簡単に識別できます。

すべてのシステムでアポトーシスを定義する単一パラメータがないため、アポトーシスを確実に検出するために、複数の測定を組み合わせて使用することを強く推奨します。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

励起／発光SYTOX AADvanced：546/647
CellEvent Caspase 3/7 Green Detection Reagent：511/533

フローサイトメーターレーザーライン488

使用対象（アプリケーション）フローサイトメトリー

使用対象 (装置)Attune™アコースティックフォーカシングサイトメーター、フローサイトメーター

標識タイプその他の標識または色素

標識または色素CellEvent™ Caspase 3/7 Green Detection Reagent、SYTOX AADvanced死細胞染色

製品ラインCellEvent

製品タイプCaspase Assay Kit

数量20アッセイ

出荷条件湿氷

検出法蛍光

フォーマットキット

Unit SizeEach

組成および保存条件

1バイアルのCellEvent™ Caspase 3/7 Green Detection Reagent、1バイアルのSYTOX AADvanced死細胞染色剤、1バイアルのDMSOを含みます。

すべてのバイアルは、-20℃以下で直立した状態で保存し、乾燥して遮光します。

よくあるご質問（FAQ）

Can I use CellEvent Caspase-3/7 Green Detection Reagent with fixed samples?

No, it must be applied to live cell populations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the CellEvent Caspase-3/7 Green Detection Reagent, may I stain cells with nucleic acid stains?

Yes, you may use SYTOX AADvanced Dead Cell Stain that is included in the CellEvent Caspase-3/7 Green Ready Flow Reagent (Cat. No. R37167) and in the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Cat. No. C10427, C10740). You may also try using other nucleic acid stains in the red or far red channels for staining cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the CellEvent Caspase-3/7 Green Detection Reagent, may I multiplex with fluorescent immunolabeling?

Yes. You can apply surface labels or other reagents. We recommend applying the surface labels or other reagents first.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the CellEvent Caspase-3/7 Green Detection Reagent fixable?

The CellEvent Caspase-3/7 Green Detection Reagent is not covalently attached to any cellular component and may be lost upon fixation. For flow cytometry applications, after applying the reagent, you should examine without washing or fixation. For imaging applications, the CellEvent product signal may be retained after fixation in 3.7% formaldehdye for 15 min.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I fix my CellEvent Caspase 3/7 - labeled cells?

For imaging applications, we recommend fixing with 3.7% formaldehyde at room temperature for 15 mins. After fixation, cells can be imaged for up to 24 hrs, but the signal will gradually degrade over time. For flow cytometry applications, fixation is not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit, 20 Assays FAQs

引用および参考文献 (7)

引用および参考文献

Abstract

Clumping and Viability of Bone Marrow Derived Mesenchymal Stromal Cells under Different Preparation Procedures: A Flow Cytometry-Based In Vitro Study.

Authors:Cui LL, Kinnunen T, Boltze J, Nystedt J, Jolkkonen J

Journal:Stem Cells Int

PubMed ID:27022399

'Complications of microocclusions have been reported after intra-arterial delivery of mesenchymal stromal cells. Hence, quantification and efficient limitation of cell clumps in suspension before transplantation is important to reduce the risk. We used a flow cytometry-based pulse-width assay to assess the effects of different cell suspension concentrations (0.2-2.0 × 10(6)/mL), ... More

New flow cytometry approaches in equine andrology.

Authors:Peña FJ, Ortega Ferrusola C, Martín Muñoz P

Journal:Theriogenology

PubMed ID:27160445

Flow cytometry is currently recognized as a robust tool for the evaluation of sperm quality and function. However, within equine reproduction, this technique has not reached the sophistication of other areas of biology and medicine. In recent years, more sophisticated flow cytometers have been introduced in andrology laboratories, and the ... More

Avenanthramide-C reduces the viability of MDA-MB-231 breast cancer cells through an apoptotic mechanism.

Authors:Hastings J, Kenealey J

Journal:Cancer Cell Int

PubMed ID:29075150

Avenanthramides (AVN) are a relatively unstudied family of phytochemicals that could be novel chemotherapeutics. These compounds, found in oats, are non-toxic to healthy cells and have been shown to reduce viability of human colon and liver cancers in vitro. However, these studies do not elucidate a molecular mechanism for individual ... More

Comparison of

Authors:Opydo-Chanek M, Sladowska K, Blicharski K, Mikeš J, Fedorocko P, Niemeyer U, Mazur L

Journal:Anticancer Res

PubMed ID:29061820

The oxazaphosphorines, ifosfamide and cyclophosphamide, represent a class of alkylating agents. The aim of the present in vitro study was to compare antileukemic activity of 4-hydroperoxyifosfamide (4-OOH-IF) and 4-hydroperoxycyclophosphamide (4-OOH-CP). ... More

Liposomal encapsulation of silver nanoparticles enhances cytotoxicity and causes induction of reactive oxygen species-independent apoptosis.

Authors:Yusuf A, Brophy A, Gorey B, Casey A

Journal:J Appl Toxicol

PubMed ID:29181855

Silver nanoparticles (AgNP) are one of the most widely investigated metallic NPs due to their promising antibacterial activities. In recent years, AgNP research has shifted beyond antimicrobial use to potential applications in the medical arena. This shift coupled with the extensive commercial applications of AgNP will further increase human exposure ... More

View all CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit, 20 Assays citations