CyQUANT™ Cell Proliferation Assays

Citations & References (15)

Invitrogen™

CyQUANT™ Cell Proliferation Assays

Name: CyQUANT™ Cell Proliferation Assays
SKU: C35006
Price: 93100 JPY

CyQUANT™ NF細胞増殖アッセイは、集団中の細胞をカウントし、マイクロプレートフォーマットで増殖を測定するための迅速かつ高感度な方法を提供します。CyQUANT™ NF細胞増殖アッセイの特長：•MTTやalamarBlue™アッセイよりも高感度• ウェルあたり100～20,000細胞の直線的検出範囲（96ウェルマイクロプレート）• アッセイは1時間で完了増殖を測定する迅速かつシンプルな方法CyQUANT™詳細を見る

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表示を変更

製品番号（カタログ番号）	細胞タイプ	数量
C35006	NF Cell	1000 Assays
C35011	Direct Cell	10 Microplates
C35013	Direct Red Cell	10個のマイクロプレート
C35012	Direct Cell	100 Microplates
C35007	NF Cell	200 Assays
C7026	For cells in culture	1000 Assays
C7027	Cell Lysis Buffer	50 mL

7 オプション

製品番号（カタログ番号） C35006

価格（JPY）

93,100

Each

カートに追加

細胞タイプ:

NF Cell

数量:

1000 Assays

価格（JPY）

93,100

Each

カートに追加

CyQUANT™ NF細胞増殖アッセイは、集団中の細胞をカウントし、マイクロプレートフォーマットで増殖を測定するための迅速かつ高感度な方法を提供します。

CyQUANT™ NF細胞増殖アッセイの特長：

•MTTやalamarBlue™アッセイよりも高感度
• ウェルあたり100～20,000細胞の直線的検出範囲（96ウェルマイクロプレート）
• アッセイは1時間で完了

増殖を測定する迅速かつシンプルな方法
CyQUANT™ NF細胞増殖アッセイでは、細胞溶解、長時間のインキュベーション、放射能、細胞からの染色の除去は不要です。CyQUANT™ NFアッセイでは、細胞透過性のDNA結合色素を細胞膜透過処理試薬と組み合わせて使用することで、オリジナルのCyQUANT™細胞増殖アッセイの凍結融解ステップが不要になります。CyQUANT™ NF細胞増殖アッセイキットは、96ウェルまたは384ウェルのマイクロプレートフォーマットで使用でき、より小さなサンプルサイズ用の200アッセイキット（C35007）と、ハイスループットアプリケーション用の1000アッセイキット（C35006）の2種類の構成で使用できます。

alamarBlue™はTREK Diagnostic Systemsの登録商標です。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

細胞タイプNF Cell

検出法蛍光

染色剤タイプその他の標識または色素

励起／発光480/520 nm

フォーマット384ウェルプレート、96ウェルプレート

数量1000 Assays

出荷条件室温

使用対象（アプリケーション）増殖アッセイ

使用対象 (装置)マイクロプレートリーダー, HTS Reader

製品ラインCyQUANT

製品タイプ細胞増殖アッセイ

Unit SizeEach

組成および保存条件

•CyQUANT™ NF dye reagent
•Dye delivery reagent
•5X HBSS buffer (Hank’s balanced salt solution)

It is possible to observe precipitate in HBSS buffer. The precipitate does not have an impact on the use of this kit, nor the results that are gathered from its use.

よくあるご質問（FAQ）

What is the difference between the original CyQuant assay and the CyQuant NF assay?

The original CyQUANT assay provides sensitive detection of cells over a 1000-fold linear dynamic range. In this assay, a freeze-thaw cell lysis step is required to facilitate the interaction of the CyQUANT GR dye with DNA. The CyQUANT NF assay avoids this freeze-thaw step by using a DNA binding dye in combination with a plasma membrane permeabilization reagent. The CyQUANT NF protocol requires only aspiration of growth medium (for adherent cells), replacement with dye binding solution, incubation for 30-60 minutes, and then measurement of fluorescence in a microplate reader. The assay is designed to produce a linear analytical response from at least 100-20,000 cells per well in most cell lines in a 96-well microplate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you have spectral data for the CyQUANT NF Cell Proliferation Assay kit?

When CyQUANT NF dye is bound to DNA, the approximate excitation/emission is 480/520 nm. It can be detected using standard GFP or FITC filter settings.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer any products to measure neuronal cell health?

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (15)

引用および参考文献

Abstract

KIF14 messenger RNA expression is independently prognostic for outcome in lung cancer.

Authors:Corson TW,Zhu CQ,Lau SK,Shepherd FA,Tsao MS,Gallie BL

Journal:Clinical cancer research : an official journal of the American Association for Cancer Research

PubMed ID:17545527

Functional symbiosis between endothelium and epithelial cells in glomeruli.

Authors:Hirschberg R, Wang S, Mitu GM,

Journal:Cell Tissue Res

PubMed ID:17999087

'In some capillary beds, pericytes regulate endothelial growth. Capillaries with high filtration capacity, such as those in renal glomeruli, lack pericytes. Glomerular endothelium lies adjacent to visceral epithelial cells (podocytes) that are anchored to and cover the anti-luminal surface of the basement membrane. We have tested the hypothesis that podocytes ... More

Flex-Hets differentially induce apoptosis in cancer over normal cells by directly targeting mitochondria.

Authors:Liu T, Hannafon B, Gill L, Kelly W, Benbrook D

Journal:Mol Cancer Ther

PubMed ID:17575110

'Flex-Het drugs induce apoptosis in multiple types of cancer cells, with little effect on normal cells. This apoptosis occurs through the intrinsic mitochondrial pathway accompanied by generation of reactive oxygen species (ROS). The objective of this study was to determine if direct or indirect targeting of mitochondria is responsible for ... More

Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-responsive core-shell nanoparticles.

Authors:Hu Y, Litwin T, Nagaraja AR, Kwong B, Katz J, Watson N, Irvine DJ,

Journal:Nano Lett

PubMed ID:17887715

'Polycations that absorb protons in response to the acidification of endosomes can theoretically disrupt these vesicles via the "proton sponge" effect. To exploit this mechanism, we created nanoparticles with a segregated core-shell structure for efficient, noncytotoxic intracellular drug delivery. Cross-linked polymer nanoparticles were synthesized with a pH-responsive core and hydrophilic ... More

Decrease of endogenous vascular endothelial growth factor may not affect glioma cell proliferation and invasion.

Authors:Hong X, Jiang F, Kalkanis SN, Zhang ZG, Zhang X, Zheng X, Mikkelsen T, Jiang H, Chopp M

Journal:J Exp Ther Oncol

PubMed ID:17552362

'Vascular endothelial growth factor (VEGF) is abundantly produced by glioma cells especially glioblastoma, the most malignant form of astrocytoma. VEGF, a well known angiogenic factor, acts in a paracrine fashion on endothelial cells to develop tumor vasculature. However, recent studies have found that several tumor cells express VEGF receptors, and ... More

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