CountBright™ Absolute Counting Beads, for flow cytometry

Citations & References (7)

Invitrogen™

CountBright™ Absolute Counting Beads, for flow cytometry

製品番号（カタログ番号）	数量
C36950	5 mL

製品番号（カタログ番号） C36950

価格（JPY）

77,100

お問い合わせください ›

CountBright™絶対計数ビーズは、広範囲の励起波長および発光波長（励起波長UV～635 nm、発光波長385～800 nm）にわたって明るく蛍光を発するミクロスフェアのキャリブレーション済み懸濁液です。CountBright™絶対計数ビーズを細胞サンプルと混合し、フローサイトメトリーで分析します。ビーズイベントと細胞イベントの比率を比較することで、サンプル中の細胞の絶対数を計算できます。CountBright™ビーズは試験サンプル中に混合されるため、このシングルプラットフォーム法での絶対細胞数は、マルチプラットフォーム試験で測定した細胞濃度よりも正確で、複雑性が低くなります。CountBright™絶対計数ビーズは、洗浄／溶解をおこなっていない全血など、あらゆる種類のサンプルに使用できます。

すべてのフローサイトメトリー細胞カウントビーズに関する情報を見る。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

直径（メートル法）7 μm

発光任意のレーザー（UV～633/635）

励起波長域UV∼635/385∼800 nm

使用対象 (装置)フローサイトメーター

フォーマット溶液

テスト数100

数量5 mL

出荷条件室温

製品ラインCountBright

製品タイプ細胞カウントビーズ

Unit SizeEach

組成および保存条件

CountBright™絶対計数ビーズ（5 mL）1本入りです。冷蔵庫（2℃～8℃）に保存し、遮光してください。

よくあるご質問（FAQ）

With the CountBright Plus Absolute Counting Beads/CountBright Absolute Counting Beads, instead of a tight cluster of beads on the scatter plot, I am seeing the beads cover a broad area. What is causing this?

Prior to taking an aliquot, make certain to thoroughly mix the beads. This may also be caused if the beads were ever frozen or the stock solution has any microbial contamination.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which buffers can I use with the CountBright Plus Absolute Counting Beads and CountBright Absolute Counting Beads?

You may use any physiological buffer or media in a pH range from pH 4 to 10. Avoid extremes of pH and high salt concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How are CountBright Plus Absolute Counting Beads different from CountBright Absolute Counting Beads?

Here are the differences:

CountBright Plus Absolute Counting Beads:
Bead Size: 4 µm
Excitation Range: 385 - 810 nm
Emission Range: 385 - 860 nm

CountBright Absolute Counting Beads:
Bead Size: 7 µm
Excitation Range: 350 - 635 nm
Emission Range: 350 - 810 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When using the Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry, some cells seem to have both red and green signal. Are these cells dead or alive or dying?

The green dye in the kit will label all the cells as it is a cell-permeant nucleic acid stain. The red dye is not cell permeant, and will only stain the cells with compromised membranes (dead cells). Therefore, any cells with red signal will be considered dead. It is possible that you will have some cells that are only red, some that are red and green, and some that are only green. Sometimes the red dye will displace the green dye. In any case, any red cells are dead.

Also, the green dye emission may bleed through into the red channel. Do a single-color staining and examine under both green and red filter sets to determine the level of bleedthrough. To avoid this bleedthrough, use a lower concentration of dye, and, if possible, use narrow bandpass filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How can I count my bacteria by flow cytometry?

There are several options. We have two fluorescence based kits that are useful for bacterial counting: Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856) and Bacteria Counting kit, for flow cytometry (Cat. No. B7277). Another option is the Flow Cytometry Sub-micron Particle Size Reference Kit (Cat. No. F13839).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CountBright™ Absolute Counting Beads, for flow cytometry FAQs

引用および参考文献 (7)

引用および参考文献

Abstract

An impaired transendothelial migration potential of chronic lymphocytic leukemia (CLL) cells can be linked to ephrin-A4 expression.

Authors:Trinidad EM, Ballesteros M, Zuloaga J, Zapata A, Alonso-Colmenar LM,

Journal:Blood

PubMed ID:19828693

'Chronic lymphocytic leukemia (CLL) cell migration into lymphoid tissues is an important aspect of the pathobiology of this disease. Here, we investigated the role of ephrin-A4 (EFNA4) in the transendothelial migration (TEM) capacity of CLL and normal B cells through interacting with endothelial EphA2 (erythropoietin-producing hepatocellular carcinoma). CLL cells showed ... More

Analysis of microRNA and protein transfer by exosomes during an immune synapse.

Authors:Villarroya-Beltri C, Gutiérrez-Vázquez C, Sánchez-Madrid F, Mittelbrunn M,

Journal:

PubMed ID:23719941

'Immune cells release microRNA-containing exosomes that can be taken up by recipient cells. Exosomes can thus act as mediators of cell-cell communication through direct exchange of genetic material between cells. Exosome-mediated transfer of miRNAs between T cells and antigen-presenting cells (APCs) can take place over long distances. Our work has ... More

Inhibition of NF-?B-mediated signaling by the cyclin-dependent kinase inhibitor CR8 overcomes prosurvival stimuli to induce apoptosis in chronic lymphocytic leukemia cells.

Authors:Cosimo E, McCaig AM, Carter-Brzezinski LJ, Wheadon H, Leach MT, Le Ster K, Berthou C, Durieu E, Oumata N, Galons H, Meijer L, Michie AM,

Journal:Clin Cancer Res

PubMed ID:23532892

Chronic lymphocytic leukemia (CLL) is currently incurable with standard chemotherapeutic agents, highlighting the need for novel therapies. Overcoming proliferative and cytoprotective signals generated within the microenvironment of lymphoid organs is essential for limiting CLL progression and ultimately developing a cure. We assessed the potency of cyclin-dependent kinase (CDK) inhibitor CR8, ... More

PKCa negatively regulates in vitro proplatelet formation and in vivo platelet production in mice.

Authors:Williams CM, Harper MT, Poole AW,

Journal:Platelets

PubMed ID:23402219

Proplatelet formation is a part of the intricate process by which platelets are generated by their precursor cell, the megakaryocyte. The processes that drive megakaryocyte maturation and platelet production are however still not well understood. The protein kinase C (PKC) family of serine/threonine kinases has been demonstrated as an important ... More

CD26 inhibition enhances perfusion recovery in ApoE-/-mice.

Authors:Haverslag RT, de Groot D, Grundmann S, Meder B, Goumans MJ, Pasterkamp G, Hoefer IE, de Kleijn DP,

Journal:Curr Vasc Pharmacol

PubMed ID:23391419

The adaptive growth of blood vessels is important to prevent tissue loss following arterial occlusion. Extravasation of monocytes is essential for this process. The peptidase CD26 targets SDF-1 alpha, a chemokine regulating monocyte trafficking. We hypothesized that blocking SDF-1 alpha inactivation, using a commercially available CD26 inhibitor, accelerates perfusion recovery ... More

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