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Coelenterazine h
Citations & References (26)
Invitrogen™

Coelenterazine h

セレンテラジンhは、複数の合成セレンテラジン類似体の1つであり、エクオリン複合体上でさまざまなCa2+親和性とスペクトル特性を与えます。天然のセレンテラジンと同様に、これらの5種類の新しい誘導体は、in vivoおよびin vitroでエクオリン複合体の再構成に使用できます。カルシウム、カリウム詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
C6780
または、製品番号C-6780
250 μg
製品番号（カタログ番号） C6780
または、製品番号C-6780
価格（JPY）
42,600
Online offer
Ends: 25-Sep-2026
60,900
割引額 18,300 (30%)
Each
数量:
250 μg
セレンテラジンhは、複数の合成セレンテラジン類似体の1つであり、エクオリン複合体上でさまざまなCa2+親和性とスペクトル特性を与えます。天然のセレンテラジンと同様に、これらの5種類の新しい誘導体は、in vivoおよびin vitroでエクオリン複合体の再構成に使用できます。

カルシウム、カリウム、pH、膜電位インジケーターなどのイオンインジケーターの詳細をご覧ください›

研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出法バイオ発光
染色剤タイプタンパク質ベース
数量250 μg
出荷条件室温
使用対象 (装置)ルミノメーター(マイクロプレート), 発光計(チューブベース)
製品タイプCoelenterazine h
Unit SizeEach
組成および保存条件
フリーザー(-5°C~-30°C)に保存し、遮光してください。

引用および参考文献 (26)

引用および参考文献
Abstract
Intracellular Ca2+ signals in Dictyostelium chemotaxis are mediated exclusively by Ca2+ influx.
Authors:Nebl T, Fisher PR
Journal:J Cell Sci
PubMed ID:9427292
'We measured folate- and cAMP-induced changes in cytoplasmic free calcium concentration ([Ca2+]i) using recombinant aequorin reconstituted in living Dictyostelium cells with coelenterazine-h. The resulting semi-synthetic protein displayed increased sensitivity to Ca2+ allowing accurate measurement of chemoattractant-induced transients at low resting levels. Both folate- and cAMP-induced Ca2+ responses were developmentally regulated, ... More
Quantitative assessment of beta 1- and beta 2-adrenergic receptor homo- and heterodimerization by bioluminescence resonance energy transfer.
Authors:Mercier JF, Salahpour A, Angers S, Breit A, Bouvier M
Journal:J Biol Chem
PubMed ID:12244098
'Quantitative bioluminescence resonance energy transfer (BRET) analysis was applied to the study of beta(1)- and beta(2)-adrenergic receptor homo- and heterodimerization. To assess the relative affinity between each of the protomers, BRET saturation experiments were carried out in HEK-293T cells. beta(1)- and beta(2)-adrenergic receptors were found to have similar propensity to ... More
The use of Renilla luciferase, Oplophorus luciferase, and apoaequorin as bioluminescent reporter protein in the presence of coelenterazine analogues as substrate.
Authors:Inouye S, Shimomura O
Journal:Biochem Biophys Res Commun
PubMed ID:9144537
'To investigate the use of various luciferases as reporter protein, the substrate specificity of recombinant Renilla luciferase, Oplophorus luciferase and recombinant apoaequorin was examined using 23 kinds of coelenterazine analogues as the substrate. The intensity of luminescence was generally highest with Oplophorus luciferase and lowest with apoaequorin, but varied widely ... More
Characterization of coelenterazine analogs for measurements of Renilla luciferase activity in live cells and living animals.
Authors:Zhao H, Doyle TC, Wong RJ, Cao Y, Stevenson DK, Piwnica-Worms D, Contag CH
Journal:Mol Imaging
PubMed ID:15142411
'In vivo imaging of bioluminescent reporters relies on expression of light-emitting enzymes, luciferases, and delivery of chemical substrates to expressing cells. Coelenterazine (CLZN) is the substrate for a group of bioluminescent enzymes obtained from marine organisms. At present, there are more than 10 commercially available CLZN analogs. To determine which ... More
Imaging [Ca2+]i with aequorin using a photon imaging detector.
Authors:Miller AL, Karplus E, Jaffe LF
Journal:Methods Cell Biol
PubMed ID:8201982
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