Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR.
Authors:Suzuki H, Murakami A, Yoshida K,
Journal:Appl Environ Microbiol
PubMed ID:22885745
'Counterselection systems facilitate marker-free genetic modifications in microbes by enabling positive selections for both the introduction of a marker gene into the microbe and elimination of the marker from the microbe. Here we report a counterselection system for Geobacillus kaustophilus HTA426, established through simultaneous disruption of the pyrF and pyrR ... More
PAR2 exerts local protection against acute pancreatitis via modulation of MAP kinase and MAP kinase phosphatase signaling.
Authors:Namkung W, Yoon JS, Kim KH, Lee MG,
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:18755806
'During acute pancreatitis, protease-activated receptor 2 (PAR2) can be activated by interstitially released trypsin. In the mild form of pancreatitis, PAR2 activation exerts local protection against intrapancreatic damage, whereas, in the severe form of pancreatitis, PAR2 activation mediates some systemic complications. This study aimed to identify the molecular mechanisms of ... More
Cell therapy for salivary gland regeneration.
Authors:Lin CY, Chang FH, Chen CY, Huang CY, Hu FC, Huang WK, Ju SS, Chen MH,
Journal:J Dent Res
PubMed ID:21297017
'There are still no effective therapies for hyposalivation caused by irradiation. In our previous study, bone marrow stem cells can be transdifferentiated into acinar-like cells in vitro. Therefore, we hypothesized that transplantation with bone marrow stem cells or acinar-like cells may help functional regeneration of salivary glands. Bone marrow stem ... More
Pancreatic contamination of mesenteric adipose tissue samples can be avoided by adjusted dissection procedures.
Authors:Caesar R, Drevon CA,
Journal:J Lipid Res
PubMed ID:18408254
Mesenteric adipose tissue, located in the mesenterium of the intestines, is believed to play a central role in the development of obesity-related diseases. We have found that mesenteric fat samples harvested from rodents are frequently of poor quality, exhibiting partly degraded RNA. To investigate the background for this observation, we ... More
Scanning the Corynebacterium glutamicum R genome for high-efficiency secretion signal sequences.
Authors:Watanabe K, Tsuchida Y, Okibe N, Teramoto H, Suzuki N, Inui M, Yukawa H,
Journal:Microbiology
PubMed ID:19246745
Systematic screening of secretion proteins using an approach based on the completely sequenced genome of Corynebacterium glutamicum R revealed 405 candidate signal peptides, 108 of which were able to heterologously secrete an active-form alpha-amylase derived from Geobacillus stearothermophilus. These comprised 90 general secretory (Sec)-type, 10 twin-arginine translocator (Tat)-type and eight ... More