Fura-2, AM, cell permeant (1 mM Solution in Anhydrous DMSO)

Citations & References (4405)

Invitrogen™

Fura-2, AM, cell permeant (1 mM Solution in Anhydrous DMSO)

Fura-2、AM—は、レシオメトリックでUV光励起可能な高親和性の細胞内カルシウム指示薬です。このアセトキシメチル（AM）エステルは非侵襲的な細胞内負荷に有用で、1 mgの量（F-1201）および特殊なパッケージ詳細を見る

製品番号（カタログ番号）	数量
F1225	1 mL

製品番号（カタログ番号） F1225

価格（JPY）

81,600

お問い合わせください ›

Fura-2、AM—は、レシオメトリックでUV光励起可能な高親和性の細胞内カルシウム指示薬です。このアセトキシメチル（AM）エステルは非侵襲的な細胞内負荷に有用で、1 mgの量（F-1201）および特殊なパッケージ（F-1221）で提供されています。細胞不透過性ペンタプトカリウム（F-1200）およびペンタナトリウム（F-6799）塩形態の色素をご用意しています。

カルシウム、カリウム、pH、膜電位インジケータなどのイオンインジケータの詳細をご覧ください ›

研究用途にのみご使用ください。診断目的には使用できません。

濃度1 mM

検出法蛍光

染色剤タイプ蛍光色素ベース

数量1 mL

出荷条件室温

使用対象（アプリケーション）細胞の生存率と増殖

使用対象 (装置)蛍光顕微鏡

製品タイプFura-2 AM

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The K_d value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (4405)

引用および参考文献

Abstract

Mechanisms of photoswitch conjugation and light activation of an ionotropic glutamate receptor.

Authors:Gorostiza P,Volgraf M,Numano R,Szobota S,Trauner D,Isacoff EY

Journal:Proceedings of the National Academy of Sciences of the United States of America

PubMed ID:17578923

The analysis of cell signaling requires the rapid and selective manipulation of protein function. We have synthesized photoswitches that covalently modify target proteins and reversibly present and withdraw a ligand from its binding site due to photoisomerization of an azobenzene linker. We describe here the properties of a glutamate photoswitch ... More

Simultaneous measurement of intracellular pH and Ca2+ in insulin-secreting cells by spectral imaging microscopy.

Authors:Martínez-Zaguilán R,Gurulé MW,Lynch RM

Journal:The American journal of physiology

PubMed ID:8967445

Functional implications of calcium permeability of the channel formed by pannexin 1.

Authors:Vanden Abeele F,Bidaux G,Gordienko D,Beck B,Panchin YV,Baranova AV,Ivanov DV,Skryma R,Prevarskaya N

Journal:The Journal of cell biology

PubMed ID:16908669

Although human pannexins (PanX) are homologous to gap junction molecules, their physiological function in vertebrates remains poorly understood. Our results demonstrate that overexpression of PanX1 results in the formation of Ca(2+)-permeable gap junction channels between adjacent cells, thus, allowing direct intercellular Ca(2+) diffusion and facilitating intercellular Ca(2+) wave propagation. More ... More

Rescue of vasopressin V2 receptor mutants by chemical chaperones: specificity and mechanism.

Authors:Robben JH,Sze M,Knoers NV,Deen PM

Journal:Molecular biology of the cell

PubMed ID:16267275

Because missense mutations in genetic diseases of membrane proteins often result in endoplasmic reticulum (ER) retention of functional proteins, drug-induced rescue of their cell surface expression and understanding the underlying mechanism are of clinical value. To study this, we tested chemical chaperones and sarco(endo)plasmic reticulum Ca(2+) ATPase pump inhibitors on ... More

Disruption of a single copy of the SERCA2 gene results in altered Ca2+ homeostasis and cardiomyocyte function.

Authors:Ji Y,Lalli MJ,Babu GJ,Xu Y,Kirkpatrick DL,Liu LH,Chiamvimonvat N,Walsh RA,Shull GE,Periasamy M

Journal:The Journal of biological chemistry

PubMed ID:10970890

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