FluoZin™-3, AM, cell permeant

Citations & References (43)

Invitrogen™

FluoZin™-3, AM, cell permeant

FluoZin™-3インジケータは、fluo-4に類似した構造を持つ Zn 2+選択的インジケータです。FluoZin™-3は、Zn2+結合親和性が高く、少なくとも1 μMまでは、Ca2+濃度には影響されません。また詳細を見る

製品番号（カタログ番号）	数量
F24195	100 μg

製品番号（カタログ番号） F24195

価格（JPY）

52,300

お問い合わせください ›

100 μg

FluoZin™-3インジケータは、fluo-4に類似した構造を持つ Zn 2+選択的インジケータです。FluoZin™-3は、Zn²⁺結合親和性が高く、少なくとも1 μMまでは、Ca²⁺濃度には影響されません。また、FluoZin™-3は、Zn²⁺の飽和レベルに応じて蛍光が50倍以上増加します。FluoZin™-3のイメージングアプリケーションが主流ですが、細胞ベースのマイクロプレートアッセイやフローサイトメトリープロトコルも開発されています。FluoZin™-3インジケータは、細胞透過型および細胞不透過の形態で提供されています。

カルシウム、カリウム、pH、および膜電位インジケータを含むイオンインジケータの詳細については、こちらを参照してください›

蛍光亜鉛インジケータ仕様：
•ラベル（Ex/EM）：FluoZin™-3（約494/516 nm）
• K_d（Zn²⁺）（バッファー内）：約15 nM
•凍結乾燥した製品はDMSOに溶解して使用できます
•製品は通常、細胞を含む培地に溶解したインジケーターを添加することにより、細胞にロードされます

亜鉛およびその他の金属イオンの蛍光インジケータを探します
当社は、細胞内の多価陽イオン濃度を測定する、イオンチャネルを介した金属イオン輸送の追跡する、環境サンプルを測定するための蛍光インジケータを多数提供しています。これらの製品の詳細については、『分子プローブ™ハンドブック』の「Zn²⁺およびその他の金属イオン用蛍光インジケータ—セクション19.7」を参照してください。

研究用途にのみご使用ください。ヒトまたは動物の治療もしくは診断目的には使用できません。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

染色剤タイプ亜鉛インジケータ

分子量982.85 g/mol

数量100 μg

出荷条件室温

使用対象（アプリケーション）細胞の生存率と増殖

使用対象 (装置)蛍光顕微鏡

製品ラインFluoZin

製品タイプ亜鉛インジケータ

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (43)

引用および参考文献

Abstract

Demand for Zn2+ in acid-secreting gastric mucosa and its requirement for intracellular Ca2+.

Authors:Liu J, Kohler JE, Blass AL, Moncaster JA, Mocofanescu A, Marcus MA, Blakely EA, Bjornstad KA, Amarasiriwardena C, Casey N, Goldstein LE, Soybel DI,

Journal:PLoS One

PubMed ID:21698273

'Recent work has suggested that Zn(2+) plays a critical role in regulating acidity within the secretory compartments of isolated gastric glands. Here, we investigate the content, distribution and demand for Zn(2+) in gastric mucosa under baseline conditions and its regulation during secretory stimulation. Content and distribution of zinc were evaluated ... More

The amyloid precursor protein (APP) of Alzheimer disease and its paralog, APLP2, modulate the Cu/Zn-Nitric Oxide-catalyzed degradation of glypican-1 heparan sulfate in vivo.

Authors:Cappai R, Cheng F, Ciccotosto GD, Needham BE, Masters CL, Multhaup G, Fransson LA, Mani K

Journal:J Biol Chem

PubMed ID:15677459

'Processing of the recycling proteoglycan glypican-1 involves the release of its heparan sulfate chains by copper ion- and nitric oxide-catalyzed ascorbate-triggered autodegradation. The Alzheimer disease amyloid precursor protein (APP) and its paralogue, the amyloid precursor-like protein 2 (APLP2), contain copper ion-, zinc ion-, and heparan sulfate-binding domains. We have investigated ... More

Clioquinol and pyrithione activate TRPA1 by increasing intracellular Zn2+.

Authors:Andersson DA, Gentry C, Moss S, Bevan S,

Journal:Proc Natl Acad Sci U S A

PubMed ID:19416844

'The antifungal and amoebicidal drug clioquinol (CQ) was withdrawn from the market when it was linked to an epidemic of subacute myelo-optico-neuropathy (SMON). Clioquinol exerts its anti-parasitic actions by acting as a Cu/Zn chelator and ionophore. Here we show that local injections of CQ produce mechanical hyperalgesia and cold hypersensitivity ... More

Intracellular zinc fluctuations modulate protein tyrosine phosphatase activity in insulin/insulin-like growth factor-1 signaling.

Authors:Haase H, Maret W

Journal:Exp Cell Res

PubMed ID:14644152

'Zinc is an effector of insulin/IGF-1 signaling and has insulinomimetic effects, the molecular basis of which is not understood. The present study establishes the capacity of zinc to inhibit protein tyrosine phosphatases (PTPs) as a cause for these effects and, moreover, demonstrates modulation of the insulin response by changes in ... More

Interleukin-6 regulates the zinc transporter Zip14 in liver and contributes to the hypozincemia of the acute-phase response.

Authors:Liuzzi JP, Lichten LA, Rivera S, Blanchard RK, Aydemir TB, Knutson MD, Ganz T, Cousins RJ

Journal:Proc Natl Acad Sci U S A

PubMed ID:15863613

'Infection and inflammation produce systemic responses that include hypozincemia and hypoferremia. The latter involves regulation of the iron transporter ferroportin 1 by hepcidin. The mechanism of reduced plasma zinc is not known. Transcripts of the two zinc transporter gene families (ZnT and Zip) were screened for regulation in mouse liver ... More

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