Fura Red™, AM, cell permeant
Fura Red™, AM, cell permeant
Citations & References (148)
Invitrogen™

Fura Red™, AM, cell permeant

Fura Red、AM—は可視性の高いlight 刺激性Fura-2アナログで、単一の励起グリーン蛍光カルシウム指示薬とともに使用した場合、マイクロ光度法、イメージング、またはフローサイトメトリーによる単一細胞中のCa 2+の比測定に独自の可能性を提供します。このアセトオキシメチル詳細を見る
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製品番号（カタログ番号）数量
F3020500 μg
F302110 x 50 μg
製品番号（カタログ番号） F3020
価格（JPY）
104,100
Each
お問い合わせください ›
数量:
500 μg
Fura Red、AM—は可視性の高いlight 刺激性Fura-2アナログで、単一の励起グリーン蛍光カルシウム指示薬とともに使用した場合、マイクロ光度法、イメージング、またはフローサイトメトリーによる単一細胞中のCa 2+の比測定に独自の可能性を提供します。このアセトオキシメチル（AM）エステルは非侵襲的な細胞内充填剤に有用で、1 mgの量（F-3020）もご利用いただけます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出法蛍光
染色剤タイプ蛍光色素ベース
数量500 μg
出荷条件室温
使用対象（アプリケーション）細胞の生存率と増殖
使用対象 (装置)蛍光顕微鏡, マイクロ光度計, フローサイトメーター
製品ラインFura Red
製品タイプカルシウムインジケーター
Unit SizeEach
組成および保存条件
フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Where can I find the manual for the Fura Red, AM, cell permeant (Cat. No. F3020, F3021)?

Unfortunately, we do not have a user manual for the Fura Red, AM, cell permeant (Cat. No. F3021). You can reference section 19.3 of the Molecular Probes handbook for recommendations for use.
There is also a large number of citations & references provided on the product page.

Basically, you can dissolve the product in good quality anhydrous DMSO to prepare a stock solution of 1-10 mM and use a final solution of about 1-10 µM in a physiological buffer. You can then incubate for 15 to 60 minutes, wash and then leave the cells for about 30 minutes to allow the AM group to hydrolyse before starting the assay. You can add Pluronic F-127 (e.g., Cat. No. P6866) to facilitate uptake. Store the DMSO stock solution at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (148)

引用および参考文献
Abstract
Mechanism of collagen activation in human platelets.
Authors:Roberts DE, McNicol A, Bose R
Journal:J Biol Chem
PubMed ID:14981087
The mechanism of collagen-induced human platelet activation was examined using Ca2+, Na+, and the pH-sensitive fluorescent dyes calcium green/fura red, sodium-binding benzofuran isophthalate, and 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Administration of a moderate dose of collagen (10 microg/ml) to human platelets resulted in an increase in [Ca2+](i) and platelet aggregation. The majority of this ... More
Cluster formation of inositol 1,4,5-trisphosphate receptor requires its transition to open state.
Authors:Tateishi Y, Hattori M, Nakayama T, Iwai M, Bannai H, Nakamura T, Michikawa T, Inoue T, Mikoshiba K
Journal:J Biol Chem
PubMed ID:15583010
'The inositol 1,4,5-trisphosphate (IP(3)) receptor (IP(3)R) Ca(2+) channel plays pivotal roles in many aspects of physiological and pathological events. It was previously reported that IP(3)R forms clusters on the endoplasmic reticulum when cytosolic Ca(2+) concentration ([Ca(2+)](C)) is elevated. However, the molecular mechanism of IP(3)R clustering remains largely unknown, and thus ... More
Triad formation: organization and function of the sarcoplasmic reticulum calcium release channel and triadin in normal and dysgenic muscle in vitro.
Authors:Flucher BE, Andrews SB, Fleischer S, Marks AR, Caswell A, Powell JA
Journal:J Cell Biol
PubMed ID:8245124
'Excitation-contraction (E-C) coupling is thought to involve close interactions between the calcium release channel (ryanodine receptor; RyR) of the sarcoplasmic reticulum (SR) and the dihydropyridine receptor (DHPR) alpha 1 subunit in the T-tubule membrane. Triadin, a 95-kD protein isolated from heavy SR, binds both the RyR and DHPR and may ... More
Real time fluorescence imaging of PLC gamma translocation and its interaction with the epidermal growth factor receptor.
Authors:Matsuda M, Paterson HF, Rodriguez R, Fensome AC, Ellis MV, Swann K, Katan M
Journal:J Cell Biol
PubMed ID:11331309
'The translocation of fluorescently tagged PLC gamma and requirements for this process in cells stimulated with EGF were analyzed using real time fluorescence microscopy applied for the first time to monitor growth factor receptor--effector interactions. The translocation of PLC gamma to the plasma membrane required the functional Src homology 2 ... More
Vav1 and Ly-GDI two regulators of Rho GTPases, function cooperatively as signal transducers in T cell antigen receptor-induced pathways.
Authors:Groysman M, Hornstein I, Alcover A, Katzav S
Journal:J Biol Chem
PubMed ID:12386169
'The Rho family GTPases are pivotal for T cell signaling; however, the regulation of these proteins is not fully known. One well studied regulator of Rho GTPases is Vav1; a hematopoietic cell-specific guanine nucleotide exchange factor critical for signaling in T cells, including stimulation of the nuclear factor of activated ... More
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