I am using a Champion pET vector with the BL21-AI cells. Can I use your MagicMedia Medium for expression?
Unfortunately, MagicMedia Medium will not work with the arabinose inducer. MagicMedia Medium needs an IPTG-inducible system along with an intact Lac operon.
MagicMedia Medium is an E. coli expression medium. It produces 3-10 times higher protein yield, is compatible with T7 regulated E. coli vectors that contain a functional lac operon and BL21 (DE3) strains, is simple to use, and eliminates OD monitoring/induction steps.