Filmtracer™ LIVE/DEAD™ Biofilm Viability Kit

Citations & References (14)

Invitrogen™

Filmtracer™ LIVE/DEAD™ Biofilm Viability Kit

製品番号（カタログ番号）	数量
L10316	1 kit

製品番号（カタログ番号） L10316

価格（JPY）

80,400

お問い合わせください ›

1 kit

FilmTracer™ LIVE⁄DEADバイオフィルム生存率キットは、細胞膜の完全性に基づき細菌の生存率を検証する、2色蛍光アッセイを提供します。このアッセイは、バイオフィルムコミュニティに生息するものも含め、多様な細菌属に有用であることが実証されています。LIVE⁄DEADバイオフィルム生存率キットでは、緑色蛍光の核酸染色剤であるSYTO™ 9、および赤色蛍光の核酸染色剤であるヨウ化プロピジウムの混合物を使用します。これらの染色剤は、スペクトル特性、および健康な細菌細胞への透過能がそれぞれ異なります。単独で使用する場合、SYTO™ 9染色剤は一般的に、損傷のない細胞膜を持つ細菌、細胞膜が損傷した細菌を含め、集団内のすべての細菌を標識します。一方、ヨウ化物プロピジウムは細胞膜が損傷した細菌のみを透過します。このため、両方の色素が存在する細胞ではSYTO™ 9染色蛍光が減弱します。したがって、SYTO™ 9とヨウ化物プロピルジウム染色剤が適切に混合されている場合、細胞膜が無傷の細菌は緑色蛍光で染色され、細胞膜が損傷した細菌は赤色蛍光に染色されます。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

染色剤タイプSYTO™ 9、ヨウ化プロピジウム

数量1 kit

出荷条件室温

細胞内局在細胞質&サイトゾル

Emission可視

使用対象（アプリケーション）生存率アッセイ

使用対象 (装置)共焦点顕微鏡, 蛍光顕微鏡, フローサイトメーター, マイクロプレートリーダー, 蛍光イメージャー

製品ラインFilmTracer、Molecular Probes

製品タイプBiofilm Viability Kit

Unit SizeEach

組成および保存条件

2つの成分をフリーザー（-5℃～-30℃）で保存し、遮光してください

よくあるご質問（FAQ）

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (14)

引用および参考文献

Abstract

Biofilm formations in nasopharyngeal tissues of patients with nasopharyngeal osteoradionecrosis.

Authors:Tsai YJ, Lin YC, Wu WB, Chiu PH, Lin BJ, Hao SP,

Journal:Otolaryngol Head Neck Surg

PubMed ID:23348872

'Objective Determine the presence of nasopharynx biofilms in patients with nasopharyngeal cancer (NPC) and osteoradionecrosis (ORN) and patients with NPC but no ORN. Study Design Cross-sectional study. Setting Tertiary referral medical center. Subjects and Methods We enrolled 27 patients with NPC from our outpatient clinic during January 2010 to June ... More

Comparison of methods for evaluation of the bactericidal activity of copper-sputtered surfaces against methicillin-resistant Staphylococcus aureus.

Authors:Rio L, Kusiak-Nejman E, Kiwi J, Bétrisey B, Pulgarin C, Trampuz A, Bizzini A,

Journal:Appl Environ Microbiol

PubMed ID:22983970

Bacteria can survive on hospital textiles and surfaces, from which they can be disseminated, representing a source of health care-associated infections (HCAIs). Surfaces containing copper (Cu), which is known for its bactericidal properties, could be an efficient way to lower the burden of potential pathogens. The antimicrobial activity of Cu-sputtered ... More

Fratricide is essential for efficient gene transfer between pneumococci in biofilms.

Authors:Wei H, Håvarstein LS,

Journal:Appl Environ Microbiol

PubMed ID:22706053

Streptococcus pneumoniae and a number of commensal streptococcal species are competent for natural genetic transformation. The natural habitat of these bacteria is multispecies biofilms in the human oral cavity and nasopharynx. Studies investigating lateral transfer of virulence and antibiotic resistance determinants among streptococci have shown that interspecies as well as ... More

Comparison of different live/dead stainings for detection and quantification of adherent microorganisms in the initial oral biofilm.

Authors:Tawakoli PN, Al-Ahmad A, Hoth-Hannig W, Hannig M, Hannig C,

Journal:Clin Oral Investig

PubMed ID:22821430

The aim of the present study was to investigate different fluorescence-based, two-color viability assays for visualization and quantification of initial bacterial adherence and to establish reliable alternatives to the ethidium bromide staining procedure. Bacterial colonization was attained in situ on bovine enamel slabs (n?=?6 subjects). Five different live/dead assays were ... More

Rapid Bacterial Detection during Endodontic Treatment.

Authors:

Journal:J Dent Res

PubMed ID:28530469

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