NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007

NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), <i>Contains 1 ea. LA0041, NP0004, NP0005, NP0007</i>

Invitrogen™

NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007

NuPAGE Tris-Acetate SDSバッファーキットは、トリス-アセテートゲルでの中∼大サイズのタンパク質の分離用に設計されています。このキットには以下のバッファーが含まれています。• NuPAGE Tris-Acetate SDSランニングバッファー（20X詳細を見る

製品番号（カタログ番号）	数量
LA0050	1 kit

製品番号（カタログ番号） LA0050

価格（JPY）

12,900

Online offer

Ends: 27-Mar-2026

21,600

割引額 8,700 (40%)

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1 kit

NuPAGE Tris-Acetate SDSバッファーキットは、トリス-アセテートゲルでの中∼大サイズのタンパク質の分離用に設計されています。このキットには以下のバッファーが含まれています。
• NuPAGE Tris-Acetate SDSランニングバッファー（20X、カタログ番号LA0041）
• NuPAGEサンプル還元剤（10X、250 µL、カタログ番号NP0004）
• NuPAGE抗酸化剤（カタログ番号NP0005）
• NuPAGE LDSサンプルバッファー（4X、10 mL、カタログ番号NP0007）

SDS-PAGEに使用可能なすべてのバッファーと試薬をご覧ください

研究用にのみ使用できます。診断用には使用いただけません。

ゲルタイプSDS PAGEゲル

数量1 kit

Gel CompatibilityNuPAGE™ Tris-Acetateゲル

製品ラインNuPAGE

製品タイプBuffer Kit

Unit SizeEach

組成および保存条件

NuPAGE™ Tris-Acetate SDSバッファーキット（Tris-Acetateゲル用）の内容：

•20X NuPAGE™ Tris-Acetate SDSランニングバッファー(500 ml)：保管温度4℃∼25℃

• 10X NuPAGE™サンプル還元剤(250 µl)：保管温度2℃∼8℃

• NuPAGE™抗酸化剤(15 ml)：保管温度4℃

• 4X NuPAGE™ LDSサンプルバッファー(10 ml)：4℃～25℃で保存

よくあるご質問（FAQ）

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can I use CTAB rather than SDS in my sample buffer?

No, CTAB will not work with any of our gels except for the NuPAGE Tris-Acetate gels. To use CTAB, you would need to use a running buffer of 50 mM acetic acid and 50 mM beta-alanine in equal concentrations. You would also need to switch the electrodes. Since CTAB is a cationic detergent, this would establish conditions for running a basic protein towards the anode (into the gel).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is there a reasonable replacement for the NuPAGE antioxidant for gel electrophoresis?

One can use 0.002% thioglycolic acid in the upper buffer reservoir. This is a good scavenger of free radicals. The reference to this is described by Hunkapiller et al, Methods of Enzymology, (91), 399, 1983. Caution should be taken when using this method since this compound is both toxic and expensive. In addition, the TGA must be fresh as it tends to become oxidized itself over time. Oxidized TGA will actually promote sample re-oxidation.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007 FAQs