Tris-Glycine Transfer Buffer (25X)

トリス-グリシン転写バッファー（25X）は、タンパク質をニトロセルロース、PVDF、またはナイロンメンブレンにウェスタンブロット転写するために最適化されており、後でトリス-グリシンゲルを用いて分析できます。使用法：最適な結果を得るには、このバッファーを水 /詳細を見る

製品番号（カタログ番号）	数量
LC3675	500 mL

製品番号（カタログ番号） LC3675

価格（JPY）

11,700

お問い合わせください ›

500 mL

トリス-グリシン転写バッファー（25X）は、タンパク質をニトロセルロース、PVDF、またはナイロンメンブレンにウェスタンブロット転写するために最適化されており、後でトリス-グリシンゲルを用いて分析できます。

使用法：最適な結果を得るには、このバッファーを水 / メタノール混合物で1X溶液に希釈して最終的なメタノール濃度20 %にする必要があります。

SDS-PAGEに使用可能なすべてのバッファーと試薬の一覧

研究用にのみ使用できます。診断用には使用いただけません。

濃度25倍

ゲルタイプTris-Glycineゲル

数量500 mL

出荷条件室温

Gel CompatibilityNovex™ Tris-Glycineゲル

Membrane Compatibilityニトロセルロース、PVDF、ナイロン

製品ラインNovex

製品タイプ転写バッファー

Unit SizeEach

組成および保存条件

室温保存。

よくあるご質問（FAQ）

What type of transfer buffer should be used with Invitrogen Tricine gels?

For non-sequencing applications, any transfer buffer used with Tris-Glycine gels can be used with Tricine gels including Tris-Glycine transfer buffer. For sequencing applications, the buffer should be chemically compatible with sequencing protocols. Non-glycine based transfer buffers such as the NuPAGE Transfer buffer, 1/2X TBE Transfer buffer, or CAPS Buffer can be used for N-terminal sequencing . Generally, a pH which is close to neutral is desirable to maintain gel and protein stability. High current should be avoided because it can lead to heat generation and instability.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

When blotting with the XCell II Blot Module, what would happen if I filled the outer buffer chamber with transfer buffer instead of water?

This is perfectly acceptable with the XCell II Blot Module. The liquid in the outer buffer chamber only serves as a coolant or heat sink. The reason why water is recommended is because it is a less expensive alternative.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The pH of my transfer buffer deviates from the recommended value by 0.2 units. Can I still use the buffer?

We recommend discarding the buffer and remaking it after rechecking the reagents and the water purity. We do not recommend adjusting the pH with acid or base, as this will increase the conductivity of the buffer and result in higher current during the transfer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

引用および参考文献 (2)

引用および参考文献

Abstract

Role of thymosin beta4 in tumor metastasis and angiogenesis.

Authors:Cha HJ, Jeong MJ, Kleinman HK,

Journal:J Natl Cancer Inst

PubMed ID:14625258

'BACKGROUND: Expression of the small peptide thymosin beta4 is associated with angiogenesis induction, accelerated wound healing, and the metastatic potential of tumor cells. However, little is known about the mechanism(s) by which thymosin beta4 promotes metastasis. METHODS: Northern blot analysis and immunohistochemistry were used to examine thymosin beta4 expression in ... More

Metformin prevents the development of chronic heart failure in the SHHF rat model.

Authors:Cittadini A, Napoli R, Monti MG, Rea D, Longobardi S, Netti PA, Walser M, Samà M, Aimaretti G, Isgaard J, Saccà L,

Journal:Diabetes

PubMed ID:22344560

Insulin resistance is a recently identified mechanism involved in the pathophysiology of chronic heart failure (CHF). We investigated the effects of two insulin-sensitizing drugs (metformin and rosiglitazone) in a genetic model of spontaneously hypertensive, insulin-resistant rats (SHHF). Thirty SHHF rats were randomized into three treatment groups as follows: 1) metformin ... More