SPQ (6-Methoxy-N-(3-Sulfopropyl)Quinolinium, Inner Salt)

SPQ (6-Methoxy-<i>N</i>-(3-Sulfopropyl)Quinolinium, Inner Salt)

Citations & References (139)

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SPQ (6-Methoxy-N-(3-Sulfopropyl)Quinolinium, Inner Salt)

塩化物インジケーターSPQは、吸収／最大発光約344/443を有し、拡散制限衝突消光を介してCl-を検出します。カルシウム、カリウム、pH、膜電位インジケーターなどのイオンインジケーターの詳細については、こちらを参照してください ›詳細を見る

製品番号（カタログ番号）	数量
M440	100 mg

製品番号（カタログ番号） M440

価格（JPY）

38,900

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100 mg

塩化物インジケーターSPQは、吸収／最大発光約344/443を有し、拡散制限衝突消光を介してCl-を検出します。

カルシウム、カリウム、pH、膜電位インジケーターなどのイオンインジケーターの詳細については、こちらを参照してください ›

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

染色剤タイプ蛍光色素ベース

数量100 mg

出荷条件室温

使用対象 (装置)共焦点顕微鏡, フローサイトメーター

製品タイプ塩化物インジケーター

Unit SizeEach

組成および保存条件

室温で保存し、光から保護します。

引用および参考文献 (139)

引用および参考文献

Abstract

Identification by site-directed mutagenesis of three arginines in uncoupling protein that are essential for nucleotide binding and inhibition.

Authors:ModrianskÃ½ M, Murdza-Inglis DL, Patel HV, Freeman KB, Garlid KD

Journal:J Biol Chem

PubMed ID:9312070

Primary regulation of uncoupling protein is mediated by purine nucleotides, which bind to the protein and allosterically inhibit fatty acid-induced proton transport. To gain increased understanding of nucleotide regulation, we evaluated the role of basic amino acid residues using site-directed mutagenesis. Mutant and wild-type proteins were expressed in yeast, purified, ... More

Fluorescent indicators of ion concentrations.

Authors:Tsien RY

Journal:Methods Cell Biol

PubMed ID:2538708

Efficient endocytosis of the cystic fibrosis transmembrane conductance regulator requires a tyrosine-based signal.

Authors:Prince LS, Peter K, Hatton SR, Zaliauskiene L, Cotlin LF, Clancy JP, Marchase RB, Collawn JF

Journal:J Biol Chem

PubMed ID:9920908

'We previously demonstrated that the cystic fibrosis transmembrane conductance regulator (CFTR) is rapidly endocytosed in epithelial cells (Prince, L. S., Workman, R. B., Jr., and Marchase, R. B. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 5192-5196). To determine the structural features of CFTR required for endocytosis, we prepared ... More

Estimation of intracellular chloride activity in isolated perfused rabbit proximal convoluted tubules using a fluorescent indicator.

Authors:Krapf R, Berry CA, Verkman AS

Journal:Biophys J

PubMed ID:3395662

'The methodology has been developed to measure cell chloride activity by fluorescence microscopy using the chloride-sensitive dye, 6-methoxy-1-(3-sulfonatopropyl)quinolinium (SPQ). SPQ was loaded into cells of the in vitro microperfused rabbit proximal convoluted tubule by a 10 min luminal perfusion with 20 mM SPQ at 38 degrees C. Fluorescence was excited ... More

Regulation of Cl/HCO3 exchange in gastric parietal cells.

Authors:Thomas HA, Machen TE

Journal:Cell Regul

PubMed ID:1742343

'Microspectrofluorimetry of the fluorescent indicators 2',7'-bis-(2-carboxyethyl)-5(and-6)carboxyfluorescein and 6-methoxy-N-(3-sulfopropyl)-quinolinium was used to measure intracellular pH (pHi), intracellular Cl (Cli), and transmembrane fluxes of HCO3 and Cl in single parietal cells (PC) in isolated rabbit gastric glands incubated in HCO3/CO2-buffered solutions. Steady-state pHi was 7.2 in both resting (50 microM cimetidine) and ... More

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