Search
Invitrogen
pro-Caspase 3 Recombinant Rabbit Monoclonal Antibody (23GB715)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 5
pro-Caspase 3 Antibody (MA5-54083) in ICC/IF
Immunocytochemistry analysis of pro-Caspase 3 in C2C12 cells. Nuclei were stained blue with DAPI; pro-Caspase 3 recombinant monoclonal antibody (Product # MA5-54082) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Product Details
MA5-54083
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB715
Immunogen
A synthetic peptide of human pro Caspase 3 (1-28AA).
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Apopain; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; CPP-32; Cysteine protease CPP32; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; unnamed protein product
Gene Aliases: CASP3; CPP32; CPP32B; SCA-1
UniProt ID: (Human) P42574
Entrez Gene ID: (Human) 836
protease binding
endopeptidase activity
aspartic-type endopeptidase activity
cysteine-type endopeptidase activity
cyclin-dependent protein serine/threonine kinase inhibitor activity
death receptor binding
protein binding
enzyme activator activity
peptidase activity
cysteine-type peptidase activity
phospholipase A2 activator activity
hydrolase activity
macromolecular complex binding
luteolysis
response to hypoxia
B cell homeostasis
negative regulation of cytokine production
proteolysis
apoptotic process
cellular response to DNA damage stimulus
axonal fasciculation
heart development
sensory perception of sound
learning or memory
intrinsic apoptotic signaling pathway in response to osmotic stress
response to xenobiotic stimulus
response to UV
response to wounding
response to glucose
response to metal ion
response to X-ray
regulation of macroautophagy
protein processing
hippocampus development
protein catabolic process
neuron differentiation
keratinocyte differentiation
erythrocyte differentiation
platelet formation
negative regulation of B cell proliferation
regulation of protein stability
response to cobalt ion
response to estradiol
response to lipopolysaccharide
glial cell apoptotic process
response to tumor necrosis factor
response to nicotine
intracellular signal transduction
interleukin-18-mediated signaling pathway
response to hydrogen peroxide
T cell homeostasis
positive regulation of apoptotic process
response to amino acid
positive regulation of neuron apoptotic process
fibroblast apoptotic process
cell fate commitment
negative regulation of cell cycle
negative regulation of activated T cell proliferation
neurotrophin TRK receptor signaling pathway
striated muscle cell differentiation
response to glucocorticoid
neuron apoptotic process
protein maturation
anterior neural tube closure
protein poly-ADP-ribosylation
pyroptosis
leukocyte apoptotic process
response to anesthetic
cellular response to staurosporine
apoptotic signaling pathway
intrinsic apoptotic signaling pathway
execution phase of apoptosis
regulation of synaptic vesicle cycle
positive regulation of pyroptotic inflammatory response
positive regulation of beta-amyloid formation
epithelial cell apoptotic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support