NeuroTrace™ 500/525 Green Fluorescent Nissl Stain - Solution in DMSO

Citations & References (22)

Invitrogen™

NeuroTrace™ 500/525 Green Fluorescent Nissl Stain - Solution in DMSO

Molecular Probesからのみ入手できるNeuroTrace緑色蛍光ニッスル染色剤は、神経細胞の体細胞にのみ存在するニッスル物質に結合します。NeuroTraceニッスル染色剤は蛍光に適したフィルターで可視化される明るい緑色の蛍光を発光し、クレシルバイオレット染色剤よりも何倍もの感度があります。NeuroTrace緑色蛍光ニッスル染色剤は、従来のFluoroNissl緑色色素を改良したものです。詳細を見る

製品番号（カタログ番号）	数量
N21480	1 mL

製品番号（カタログ番号） N21480

価格（JPY）

56,800

お問い合わせください ›

Molecular Probesからのみ入手できるNeuroTrace緑色蛍光ニッスル染色剤は、神経細胞の体細胞にのみ存在するニッスル物質に結合します。NeuroTraceニッスル染色剤は蛍光に適したフィルターで可視化される明るい緑色の蛍光を発光し、クレシルバイオレット染色剤よりも何倍もの感度があります。NeuroTrace緑色蛍光ニッスル染色剤は、従来のFluoroNissl緑色色素を改良したものです。

研究用にのみ使用できます。診断用には使用いただけません。

色Green

検出法蛍光

使用対象 (装置)蛍光顕微鏡

製品タイプニッスル染色

数量1 mL

出荷条件室温

細胞内局在ニッスル小体

Excitation/Emission500/525 nm

製品ラインNEUROTRACE

Unit SizeEach

組成および保存条件

フリーザー（-5～-30度）に保存し、遮光してください。

よくあるご質問（FAQ）

I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?

Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I can use the NeuroTrace Nissl stains for staining glia or other cell types. What can I do to improve the staining so that it is more selective for neurons?

Our NeuroTrace Nissl stains label the Nissl substance which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells. These dyes are not completely specific for neurons, but will selectively stain neurons based on their high level of protein synthesis. In some cases they may show staining of other cell types such as glia, so you may need to decrease the staining concentration to obtain more selective neuronal labeling. We suggest dilutions in the range of 20- to 300-fold for neuronal staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the NeuroTrace Nissl stains be used on paraffin sections?

We have only tested them on mouse brain cryosections, however, there is at least one citation describing their use on paraffin tissue sections (Michelle L. Schlief, Ann Marie Craig, and Jonathan D. Gitlin. NMDA Receptor Activation Mediates Copper Homeostasis in Hippocampal Neurons. The Journal of Neuroscience, January 5, 2005, 25(1):239 - 246).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the NeuroTrace Nissl stains?

The dyes are proprietary, however they are stains that label the Nissl substance, which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (22)

引用および参考文献

Abstract

Beyond the olfactory bulb: an odotopic map in the forebrain.

Authors:Nikonov AA, Finger TE, Caprio J

Journal:Proc Natl Acad Sci U S A

PubMed ID:16339016

'We report electrophysiological evidence that a simple odotopy, the spatial mapping of different odorants, is maintained above the level of the olfactory bulb (OB). Three classes of biologically relevant odorants for fish are processed in distinct regions of the forebrain (FB) in the channel catfish. Feeding cues, mainly amino acids ... More

JNK-mediated induction of cyclooxygenase 2 is required for neurodegeneration in a mouse model of Parkinson's disease.

Authors:Hunot S, Vila M, Teismann P, Davis RJ, Hirsch EC, Przedborski S, Rakic P, Flavell RA

Journal:Proc Natl Acad Sci U S A

PubMed ID:14704277

'Parkinson''s disease (PD) is a neurodegenerative disorder characterized by loss of dopamine-containing neurons, but the molecular pathways underlying its pathogenesis remain uncertain. Here, we show that by eliminating c-Jun N-terminal kinases (JNKs) we can prevent neurodegeneration and improve motor function in an animal model of PD. First, we found that ... More

Defects in pancreatic development and glucose metabolism in SMN-depleted mice independent of canonical spinal muscular atrophy neuromuscular pathology.

Authors:Bowerman M, Michalski JP, Beauvais A, Murray LM, DeRepentigny Y, Kothary R,

Journal:

PubMed ID:24497575

Spinal muscular atrophy (SMA) is characterized by motor neuron loss, caused by mutations or deletions in the ubiquitously expressed survival motor neuron 1 (SMN1) gene. We recently identified a novel role for Smn protein in glucose metabolism and pancreatic development in both an intermediate SMA mouse model (Smn(2B/-)) and type ... More

Cortical control of adaptation and sensory relay mode in the thalamus.

Authors:Mease RA, Krieger P, Groh A,

Journal:

PubMed ID:24748112

A major synaptic input to the thalamus originates from neurons in cortical layer 6 (L6); however, the function of this cortico-thalamic pathway during sensory processing is not well understood. In the mouse whisker system, we found that optogenetic stimulation of L6 in vivo results in a mixture of hyperpolarization and ... More

High-throughput morphometric analysis of individual neurons.

Authors:Wu CC, Reilly JF, Young WG, Morrison JH, Bloom FE

Journal:Cereb Cortex

PubMed ID:15054070

To facilitate high-throughput quantitative analysis of neuronal structure, this study optimized the diOlistic method of whole neuron labeling to examine multiple neurons in fixed brain, and optimized image acquisition parameters to preserve signal for subsequent photoconversion. Fluorescent dye-coated gold particles were successively delivered by helium-powered ejection to 250 microm thick ... More

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