NeuroTrace™ DiI Tissue-Labeling Paste

Citations & References (11)

Invitrogen™

NeuroTrace™ DiI Tissue-Labeling Paste

NeuroTrace組織ラベリングペーストは、DiI、DiO（N-22881）またはDiD（N-22882）を不活性な防水ゲルに混合したもので構成されています。このペーストは、すぐに使用可能で、針の先端を使用して生組織試料または固定組織試料に直接塗布することができます詳細を見る

製品番号（カタログ番号）	数量
N22880 または、製品番号N-22880	500 mg

製品番号（カタログ番号） N22880

または、製品番号N-22880

価格（JPY）

56,800

お問い合わせください ›

500 mg

NeuroTrace組織ラベリングペーストは、DiI、DiO（N-22881）またはDiD（N-22882）を不活性な防水ゲルに混合したもので構成されています。このペーストは、すぐに使用可能で、針の先端を使用して生組織試料または固定組織試料に直接塗布することができます。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

使用対象 (装置)蛍光顕微鏡

製品タイプ組織標識ペースト

数量500 mg

出荷条件室温

細胞内局在細胞膜&脂質

製品ラインNEUROTRACE

Unit SizeEach

組成および保存条件

室温で保存し、光から保護します。

よくあるご質問（FAQ）

I am doing retrograde neuronal labeling using the NeuroTrace DiI Paste. I apply a small glob onto the neuron of interest, but it is not adhering very well. What can I do?

This paste, which has DiI C₁₈ uniformly dispersed within a proprietary hydrophobic carrier, is commonly used to dab onto larger areas than smaller areas commonly used with crystals. Any moisture on the area the paste is applied may be the cause for lack of adherence. Apply the paste using a small flat-head spatula or toothpick and keep it in place a bit longer to ensure contact. The area may also be covered with parafilm to ensure contact as well.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I know which tracer to choose for my experiment?

Factors to consider are size of tracer, method of delivery (injection, direct application to tissue, etc.), and if the tracer needs to be fixable. Here are some links to details about the various classes of neuronal tracers we offer and how to choose between them:

Neuronal Tracing (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html)
Choosing a Tracer (https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/fluorescent-tracers-of-cell-morphology-and-fluid-flow/choosing-a-tracer.html)
Imaging Analysis (http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/bioprobes-50-journal.pdf)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (11)

引用および参考文献

Abstract

In vivo mammalian brain imaging using one- and two-photon fluorescence microendoscopy.

Authors:Jung JC, Mehta AD, Aksay E, Stepnoski R, Schnitzer MJ

Journal:J Neurophysiol

PubMed ID:15128753

'One of the major limitations in the current set of techniques available to neuroscientists is a dearth of methods for imaging individual cells deep within the brains of live animals. To overcome this limitation, we developed two forms of minimally invasive fluorescence microendoscopy and tested their abilities to image cells ... More

Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF.

Authors:Alsina B, Vu T, Cohen-Cory S

Journal:Nat Neurosci

PubMed ID:11593233

'Dynamic developmental changes in axon arbor morphology may directly reflect the formation, stabilization and elimination of synapses. We used dual-color imaging to study, in the live, developing animal, the relationship between axon arborization and synapse formation at the single cell level, and to examine the participation of brain-derived neurotrophic factor ... More

Macrophages kill T9 glioma tumor cells bearing the membrane isoform of macrophage colony stimulating factor through a phagocytosis-dependent pathway.

Authors:Jadus MR, Williams CC, Avina MD, Ly M, Kim S, Liu Y, Narasaki R, Lowell CA, Wepsic HT

Journal:J Immunol

PubMed ID:9551992

Rat T9 glioma cells transfected with the gene for the membrane isoform of macrophage-CSF (mM-CSF) but not for the secreted isoform of M-CSF were directly killed by bone marrow-derived macrophages. Macrophage-mediated cytolysis of the mM-CSF-transfected clone was blocked by using chemical inhibitors of phagocytosis such as iodoacetate, 2-deoxyglucose, gadolinium chloride, ... More

Partition of membrane probes in a gel/fluid two-component lipid system: a fluorescence resonance energy transfer study.

Authors:Loura LM, Fedorov A, Prieto M

Journal:Biochim Biophys Acta

PubMed ID:10930513

A non-ideal lipid binary mixture (dilauroylphosphatidylcholine/distearoylphosphatidylcholine), which exhibits gel/fluid phase coexistence for wide temperature and composition ranges, was studied using photophysical techniques, namely fluorescence anisotropy, lifetime and resonance energy transfer (FRET) measurements. The FRET donor, N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-dilauroylphosphatidylethanol amine, and a short-tailed FRET acceptor, 1,1'-didodecil-3,3,3',3'-tetramethylindocarbocyanine (DiIC12(3)), were shown to prefer the fluid ... More

A discrete stage of baculovirus GP64-mediated membrane fusion.

Authors:Kingsley DH, Behbahani A, Rashtian A, Blissard GW, Zimmerberg J

Journal:Mol Biol Cell

PubMed ID:10588652

Viral fusion protein trimers can play a critical role in limiting lipids in membrane fusion. Because the trimeric oligomer of many viral fusion proteins is often stabilized by hydrophobic 4-3 heptad repeats, higher-order oligomers might be stabilized by similar sequences. There is a hydrophobic 4-3 heptad repeat contiguous to a ... More

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