NuPAGE™ MOPS SDS Running Buffer (20X)
NuPAGE™ MOPS SDS Running Buffer (20X)
Citations & References (1)
Invitrogen™

NuPAGE™ MOPS SDS Running Buffer (20X)

NuPAGE MOPS SDS泳動バッファー（20X）は、NuPAGE Novex Bis-Trisゲルでのタンパク質泳動用に調製されています。中～大サイズのタンパク質の分離に推奨されます。タンパク質の分離を最適化する適切なバッファーをご使用ください詳細を見る
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製品番号（カタログ番号）数量
NP0001025 L
NP0001500 mL
製品番号（カタログ番号） NP000102
価格（JPY）
128,200
Each
お問い合わせください ›
数量:
5 L
NuPAGE MOPS SDS泳動バッファー（20X）は、NuPAGE Novex Bis-Trisゲルでのタンパク質泳動用に調製されています。中～大サイズのタンパク質の分離に推奨されます。

タンパク質の分離を最適化する適切なバッファーをご使用ください
NuPAGE MES SDS泳動バッファーおよびNuPAGE MOPS SDS泳動バッファーはどちらもNuPAGE Bis-Trisゲルで使用できます。MESのpKaはMOPSよりも低いため、MES SDS泳動バッファーはMOPS SDS泳動バッファーよりも高速です。イオン移動の差異はスタッキングに影響することから、これらのバッファーでのタンパク質の分離範囲に差が生じます。MOPSバッファーを使用すると、MESバッファーを使用する場合よりもタンパク質の泳動速度を遅くすることができます。

MESを使用した場合とMOPSを使用した場合のNuPAGE Bis-Trisゲルでのタンパク質移動の比較

SDS-PAGEに使用可能なすべてのバッファーと試薬の一覧

研究用にのみ使用できます。診断用には使用いただけません。
仕様
化学物質名または材質Running Buffer
組成50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.7, 0.01-0.09% N,N-dimethylformamide
推奨保存方法Contents: NuPAGE™ MOPS SDS Running Buffer (20X)
Storage: +4°C to 25°C
濃度20X
物理的フォームLiquid
製品ラインNuPAGE
数量5 L
pH7.7
Unit SizeEach

よくあるご質問（FAQ）

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can I use NuPAGE Bis-Tris gels with NuPAGE MOPS or MES Running Buffer prepared without SDS for electrophoresis under native conditions?

We do not recommend using NuPAGE Bis-Tris gels with NuPAGE MOPS or MES Running Buffer prepared without SDS for electrophoresis under native conditions. This buffer system may generate excessive heat, resulting in poor band resolution. Further, the protein of interest may not migrate very well in a neutral pH environment if it is not charged.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ MOPS SDS Running Buffer (20X), 5 L FAQs

引用および参考文献 (1)

引用および参考文献
Abstract
Heat shock protein-70 expressed on the surface of cancer cells binds parathyroid hormone-related protein in vitro.
Authors:Grzesiak JJ, Smith KC, Chalberg C, Truong C, Burton DW, Deftos LJ, Bouvet M,
Journal:Endocrinology
PubMed ID:15878959
Recent studies have shown that the functions of PTH-related protein (PTHrP) and its derived peptides cannot be attributed solely to PTH/PTHrP receptor binding. The present study focused on the identification of other proteins that might bind PTHrP at the cell surface. Using affinity chromatography, we applied extracts of cell-surface biotinylated ... More