NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)
NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)
Citations & References (1)
Invitrogen™

NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)

NuPAGE MOPS SDSバッファーキットは、NuPAGE Bis-Trisゲルでの中∼大サイズのタンパク質の分離用に設計されており、以下のバッファーを含んでいます。• NuPAGE MOPS SDSランニングバッファー詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
NP00501 kit
製品番号（カタログ番号） NP0050
価格（JPY）
22,600
Each
お問い合わせください ›
数量:
1 kit
NuPAGE MOPS SDSバッファーキットは、NuPAGE Bis-Trisゲルでの中∼大サイズのタンパク質の分離用に設計されており、以下のバッファーを含んでいます。
• NuPAGE MOPS SDSランニングバッファー（20X、500 mL、カタログ番号NP0001）
• NuPAGEサンプル還元剤（10X、250 µL、カタログ番号NP0004）
• NuPAGE抗酸化剤（カタログ番号NP0005）
• NuPAGE LDSサンプルバッファー（4X、10 mL、カタログ番号NP0007）

NuPAGE Bis-TrisゲルでMESおよびMOPを使用してタンパク質の移動パターンを比較

SDS-PAGEに利用可能なすべてのバッファーと試薬を確認

研究用途にのみご使用ください。診断目的には使用できません。
仕様
ゲルタイプSDS PAGEゲル
数量1 kit
出荷条件室温
Gel CompatibilityNuPAGE™ Bis-Trisゲル
製品ラインNuPAGE
製品タイプ泳動バッファー
Unit SizeEach
組成および保存条件
付属品
• NuPAGE™ MOPS SDSランニングバッファー(20X、500 ml)
• NuPAGE™サンプル還元剤(10X、250 µl、500mMジチオトリトールを含む)
• NuPAGE™抗酸化剤（15mL）
•NuPAGE™ LDSサンプルバッファー（10 ml）
それぞれの保管温度は4℃です。NuPAGE™ LDSサンプルバッファーを使用する前に25℃に温めてください。

よくあるご質問（FAQ）

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels) FAQs

引用および参考文献 (1)

引用および参考文献
Abstract
Heat shock protein-70 expressed on the surface of cancer cells binds parathyroid hormone-related protein in vitro.
Authors:Grzesiak JJ, Smith KC, Chalberg C, Truong C, Burton DW, Deftos LJ, Bouvet M,
Journal:Endocrinology
PubMed ID:15878959
Recent studies have shown that the functions of PTH-related protein (PTHrP) and its derived peptides cannot be attributed solely to PTH/PTHrP receptor binding. The present study focused on the identification of other proteins that might bind PTHrP at the cell surface. Using affinity chromatography, we applied extracts of cell-surface biotinylated ... More