Oregon Green™ 488 BAPTA-5N, Hexapotassium Salt, cell impermeant

Citations & References (46)

Invitrogen™

Oregon Green™ 488 BAPTA-5N, Hexapotassium Salt, cell impermeant

細胞不透過性の可視光—励起性、低親和性Ca2+インジケーター、Oregon Green™ 488 BAPTA-5N、六カリウム塩は、Ca2+のKd約20 μM、吸収／最大発光約492/517詳細を見る

製品番号（カタログ番号）	数量
O6812	500 μg

製品番号（カタログ番号） O6812

価格（JPY）

70,800

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500 μg

細胞不透過性の可視光—励起性、低親和性Ca²⁺インジケーター、

Oregon Green™ 488 BAPTA-5N、六カリウム塩
は、Ca²⁺のK_d約20 μM、吸収／最大発光約492/517 nmを有しています。

カルシウム、カリウム、pH、膜電位インジケーターなどのイオンインジケーターの詳細については、こちらを参照してください ›

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

染色剤タイプ蛍光色素ベース

数量500 μg

出荷条件室温

使用対象 (装置)蛍光顕微鏡, フローサイトメーター, マイクロプレートリーダー

製品ラインOregon Green

製品タイプカルシウムインジケーター

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

引用および参考文献 (46)

引用および参考文献

Abstract

Presynaptic Ca(2+) influx at the inhibitor of the crayfish neuromuscular junction: a photometric study at a high time resolution.

Authors:Vyshedskiy A, Lin JW

Journal:J Neurophysiol

PubMed ID:10634895

'Presynaptic calcium influx at the inhibitor of the crayfish neuromuscular junction was investigated by measuring fluorescence transients generated by calcium-sensitive dyes. This approach allowed us to correlate presynaptic calcium influx with transmitter release at a high time resolution. Systematic testing of the calcium indicators showed that only low-affinity dyes, with ... More

Light dependence of calcium and membrane potential measured in blowfly photoreceptors in vivo.

Authors:Oberwinkler J, Stavenga DG

Journal:J Gen Physiol

PubMed ID:9689022

'Light adaptation in insect photoreceptors is caused by an increase in the cytosolic Ca2+ concentration. To better understand this process, we measured the cytosolic Ca2+ concentration in vivo as a function of adapting light intensity in the white-eyed blowfly mutant chalky. We developed a technique to measure the cytosolic Ca2+ ... More

Stimulation-evoked increases in cytosolic [Ca(2+)] in mouse motor nerve terminals are limited by mitochondrial uptake and are temperature-dependent.

Authors:David G, Barrett EF

Journal:J Neurosci

PubMed ID:11007886

'Increases in cytosolic [Ca(2+)] evoked by trains of action potentials (20-100 Hz) were recorded from mouse and lizard motor nerve terminals filled with a low-affinity fluorescent indicator, Oregon Green BAPTA 5N. In mouse terminals at near-physiological temperatures (30-38 degrees C), trains of action potentials at 25-100 Hz elicited increases in ... More

Direct measurement of SR release flux by tracking 'Ca2+ spikes' in rat cardiac myocytes.

Authors:Song LS, Sham JS, Stern MD, Lakatta EG, Cheng H

Journal:J Physiol

PubMed ID:9769413

'1. Ca2+ release flux across the sarcoplasmic reticulum (SR) during cardiac excitation-contraction coupling was investigated using a novel fluorescence method. Under whole-cell voltage-clamp conditions, rat ventricular myocytes were dialysed with a high concentration of EGTA (4.0 mM, 150 nM free Ca2+), to minimize the residence time of released Ca2+ in ... More

Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells.

Authors:Sherwood MW, Prior IA, Voronina SG, Barrow SL, Woodsmith JD, Gerasimenko OV, Petersen OH, Tepikin AV

Journal:Proc Natl Acad Sci U S A

PubMed ID:17363470

'The intracellular activation of trypsinogen, which is both pH- and calcium-dependent, is an important early step in the development of acute pancreatitis. The cellular compartment in which trypsinogen activation occurs currently is unknown. We therefore investigated the site of intracellular trypsinogen activation by using an established cellular model of acute ... More

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