ProSightPD Software

Perform comprehensive analysis of top-down proteomics data with Thermo Scientific™ ProSightPD™ software. ProSightPD features robust proteomic search engines for proteoform characterization, including proteoforms with PTMs or truncations. ProSightPD software accommodates proteomic data generated by fragmentation techniques present in any Thermo Scientific mass spectrometer. ProSightPD software is the only proteomics software that allows the user to easily search MS/MS data against proteome databases containing the complete biological complexity found in UniProt.

• Includes five different database search modes – Sequence Tag, Single Protein, and Gene Restricted search-for exceptional intact protein characterization.
• Performs false discovery rate calculations at the protein, isoform, proteoform, and proteoform hit level.
• Processes many different types of fragmentation data, including data generated using ETD, HCD, EThcD, UVPD, ECD, IRMPD, or CID.
• Enables merging of graphical fragment maps, even those with different ion types.
• Includes proprietary ProSight containing all known post-translational modifications (PTMs), alternative splicing events and single nucleotide polymorphisms (SNPs).
• Offers more robust precursor mass analysis, with FAIMS and PTR data now readily analyzable using ProSightPD software. Both high- and low-resolution data can be processed with the Xtract and ReSpect algorithms embedded in ProSightPD software, allowing analysis of masses from a wide range of masses (∼1 kDa to >150 kDa).
• Imports XML and FASTA databases and shotgun annotates them with all possible modifications.
• Allows users to experience unparalleled, versatile visualization for reviewing search results and customizing modifications to find better result fits.