What do ProLong Gold, ProLong Diamond and ProLong Glass antifade mountant reagents contain?
They are composed of proprietary antifades and polymers in aqueous buffers.
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Can I use ProLong Gold, ProLong Diamond, or ProLong Glass mounting medium in a multi-well plate?
No. If the sample cannot be sealed with a coverslip, we recommend the use of SlowFade antifade mountant.
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What can affect sample curing of ProLong Gold-, ProLong Diamond-, and ProLong Glass-mounted samples?
Our ProLong Gold, ProLong Diamond, and ProLong Glass mounting media harden ('cure') by the evaporation of water. Placing the mounted samples in a humid or refrigerated environment would slow down the evaporation process ('curing').
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Are ProLong, ProLong Gold, ProLong Diamond, and ProLong Glass reagents compatible with DyLight dye-conjugated antibodies and streptavidin and with other organic dyes?
We have not tested ProLong, ProLong Gold, ProLong Diamond, or ProLong Glass on DyLight dye-conjugated proteins or with other organic dyes, but it is likely that they will be compatible.
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Can I use any of the ProLong, ProLong Gold, ProLong Diamond, or ProLong Glass reagents on live samples?
No. ProLong, ProLong Gold, ProLong Diamond, and ProLong Glass reagents are intended for use on fixed or fixed/permeabilized samples. Only ProLong Live Antifade Reagent (Cat Nos. P36974 and P36975) is intended for use with live cells.
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