dNTP/dUTP Mix

Thermo Scientific dNTP/dUTP Mix is a solution containing dATP, dCTP and dGTP, each at a final concentration of 2 mM and dUTP of 4 mM.ヌクレオチドの純度は99％以上で、ヌクレアーゼ活性がなく、ヒトおよび大腸菌DNAは含まれていません。ミックスを使用すると、分注ステップ数を減らし、反応セットアップエラーのリスクを低減できます。It is designed for direct use in PCR with Taq DNA Polymerase, reverse transcription, and other applications.

Highlights

• Greater than 99% purity confirmed by HPLC
• Free of human and E. coli DNA

Highly stable
• Stable for a minimum 100 freeze-thaw cycles
• Up to 95% of dNTPs remain in triphosphate form even after 7 weeks at room temperature
• Up to 90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 min at 72°C)

Application tested in:
• cDNA synthesis and RT-PCR
• Real-time PCR
• Standard PCR

Applications

• For use in all molecular biology applications, including PCR, real-time PCR & RT-PCR with Taq DNA Polymerase, cDNA synthesis, MDA, and DNA sequencing.

Note
• dNTP/dUTP Mix is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes.