Image-iT™ FX Signal Enhancer ReadyProbes™ Reagent

Citations & References (10)

Invitrogen™

Image-iT™ FX Signal Enhancer ReadyProbes™ Reagent

製品番号（カタログ番号）	数量
R37107	6 x 2.5 mL kit

製品番号（カタログ番号） R37107

価格（JPY）

42,900

お問い合わせください ›

6 x 2.5 mL kit

Image-iT™ FX Signal Enhancer ReadyProbes™ Reagentは、ストレプトアビジンまたは二次抗体のコンジュゲートを使用する二次検出中の非特異的染色によるバックグラウンド蛍光を劇的に低減します。この試薬は、蛍光プローブで染色する前に、固定および透過処理した細胞または組織サンプルを含むスライドまたはカバースリップに直接適用されます。

•簡便—ドロッパーボトルを使用して溶液を加えます
•免疫染色の他の手順に適合できます
•ほとんどの蛍光標識に有効です
•室温で安定しています—手元や細胞培養エリアに置いてください

細胞染色用のその他のReadyProbes™試薬を見る
その他の固定細胞イメージング用ツールを見る

細胞イメージングアプリケーション
Image-iT™ Fxシグナルエンハンサー試薬は、広範な蛍光色素と細胞および組織成分との非特異的な相互作用に由来するバックグラウンド染色をブロックするための非常に効果的な製品です。染色する前に、Image-iT™ FXシグナルエンハンサー試薬を固定細胞および透過細胞に適用すると、ストレプトアビジンおよび抗体複合体の蛍光結合で見られるバックグラウンド染色がほとんどなくなります。

推奨使用方法
•免疫染色の前に、Image-iT™ FXシグナルエンハンサー試薬を細胞サンプルまたは組織サンプルを含むスライドまたはカバースリップに直接適用します。
•固定／パームステップの後、洗浄してサンプルを覆うのに十分な量を加え、30分間インキュベートして通常の染色プロトコルに進みます。
•必要に応じて、Image-iT™シグナルエンハンサーReadyProbes™試薬でブロッキングした後に、追加のブロッキング手順を実行できます

研究用途にのみご使用ください。診断目的には使用できません。

フォーマットBottle(s)

製品ラインImage-iT, ReadyProbes

数量6 x 2.5 mL kit

製品タイプSignal Enhancer

溶液タイプSignal Enhancer

Unit SizeEach

組成および保存条件

6 × 2.5 mLドロッパーボトル

≦25℃で保管してください。

よくあるご質問（FAQ）

I used Image-iT FX Signal Enhancer solution to get rid of nonspecific nuclear labeling with Alexa Fluor 568 secondary antibody, but I also saw a significant reduction in my specific mitochondrial antibody labeling. Why is this and what can I do?

The Image-iT FX Signal Enhancer reduces non-specific binding of dye conjugates by blocking positively-charged areas of cells or tissues that attract negatively-charged dyes. In cells after fixation, some positively-charged structures are the nuclei and mitochondria. Thus, you would expect to see a reduction in both mitochondrial and nuclear signal. The lower signal you see afterward is the specific mitochondrial signal; the fluorescence that was lost was the non-specific labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the Image-iT FX Signal Enhancer instead of my normal blocking solution (BSA or serum)?

No. Image-iT FX Signal Enhancer is not a protein blocker, like BSA, normal serum, or other commercial antibody blockers. Use it as a separate step to block non-specific charge-based binding of dyes to cellular components.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am labeling fixed and permeabilized cultured cells with an Alexa Fluor secondary antibody. My secondary-only control is showing nuclear and mitochondrial labeling, even though I did a thorough protein blocking and tried a concentration range. What can be done to minimize this non-specific binding?

This is most likely charge-based binding due to interactions of the charge on the dyes with cellular components of opposite charge. This can be blocked by using the Image-iT FX Signal Enhancer Solution which eliminates non-specific binding due to charge. The Signal Enhancer is applied as a pre-blocking step and your regular blocking regimen should be used to limit non-specific binding due to protein-protein interactions. Signal Enhancer cannot be used on live cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am observing high background when I analyze my click-labeled samples. What is causing this and what can I do to reduce the background?

The click reaction is very selective between an azide and alkyne. No other side reactions are possible in a biological system. Any non-specific background is due to non-covalent binding of the dye to various cellular components. The Select FX Signal Enhancer is not effective at reducing non-specific charge-based binding of dyes following the click reaction; we do not recommend its use with the Click-iT detection reagents. The best method to reduce background is to increase the number of BSA washes. You should always do a no-dye or no-click reaction control under the same processing and detection conditions to verify that the background is actually due to the dye and not autofluorescence. You can also perform the complete click reaction on a carrier solvent-only, no EdU or no-EU control to verify the specificity of the click reaction signal.

Find additional tips, troubleshooting help, and resources within our Cell Viability, Proliferation, Cryopreservation, and Apoptosis Support Center.

View all Image-iT™ FX Signal Enhancer ReadyProbes™ Reagent FAQs

引用および参考文献 (10)

引用および参考文献

Abstract

Functional scaffold-free 3-D cardiac microtissues: a novel model for the investigation of heart cells.

Authors:Desroches BR, Zhang P, Choi BR, King ME, Maldonado AE, Li W, Rago A, Liu G, Nath N, Hartmann KM, Yang B, Koren G, Morgan JR, Mende U,

Journal:Am J Physiol Heart Circ Physiol

PubMed ID:22427522

To bridge the gap between two-dimensional cell culture and tissue, various three-dimensional (3-D) cell culture approaches have been developed for the investigation of cardiac myocytes (CMs) and cardiac fibroblasts (CFs). However, several limitations still exist. This study was designed to develop a cardiac 3-D culture model with a scaffold-free technology ... More

Generation of an induced pluripotent stem cell line (TRNDi008-A) from a Hunter syndrome patient carrying a hemizygous 208insC mutation in the IDS gene.

Authors:Hong J, Xu M, Li R, Cheng YS, Kouznetsova J, Beers J, Liu C, Zou J, Zheng W

Journal:Stem Cell Res

PubMed ID:31071499

'Mucopolysaccharidosis Type II (MPS II), also known as Hunter syndrome, is a rare X-linked genetic disease caused by mutations in the IDS gene encoding iduronate 2-sulfatase (I2S). This is a multisystem disorder with significant variation in symptoms. Here, we document a human induced pluripotent stem cell (iPSC) line generated from ... More

Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes.

Authors:

Journal:Nat Methods

PubMed ID:34059829

The aberrant upregulation of exon 10-inclusive SREK1 through SRSF10 acts as an oncogenic driver in human hepatocellular carcinoma.

Authors:

Journal:Nat Commun

PubMed ID:35296659

HIV-infected macrophages resist efficient NK cell-mediated killing while preserving inflammatory cytokine responses.

Authors:

Journal:Cell Host Microbe

PubMed ID:33571449

View all Image-iT™ FX Signal Enhancer ReadyProbes™ Reagent citations