CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent

Citations & References (29)

Invitrogen™

CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent

製品番号（カタログ番号）	数量
R37111	1.75 mL

製品番号（カタログ番号） R37111

価格（JPY）

42,900

お問い合わせください ›

1.75 mL

CellEvent™カスパーゼ-3/7 Green ReadyProbes™試薬は、生細胞アプリケーションおよび固定細胞アプリケーション用の活性化カスパーゼ-3/7の蛍光非洗浄指標です。カスパーゼ-3の活性化はアポトーシスの早期指標であり、CellEvent™カスパーゼ-3/7 Green試薬は細胞死することになる細胞を迅速かつ高感度に検出できます。

•シンプルで迅速な洗浄不要なプロトコルは、繊細なアポトーシスを起こしている細胞の保存に役立ちます
• 生細胞蛍光イメージングとホルムアルデヒドベースの固定法の両方に適合します
• すぐに使用できる便利なドロッパーボトル入りの液体製剤で、—希釈、重量測定、ピペット操作は不要です
• 室温で安定し、—スコープや細胞培養エリアで便利です

細胞染色用のその他のReadyProbes™試薬をご覧ください
アポトーシス用のその他のアッセイの詳細をご覧ください

細胞イメージングアプリケーション
CellEvent™カスパーゼ-3/7 Green試薬は、DNAに結合していない場合に非蛍光性である核酸結合色素にコンジュケートされる4アミノ酸ペプチド（DEVD）です。CellEvent™カスパーゼ-3/7 Green試薬は、DEVDペプチドがDNAへの色素の結合を阻害するため、本質的に非蛍光です。アポトーシスを起こしている細胞でカスパーゼ3/7を活性化すると、DEVDペプチドが開裂され、遊離色素がDNAに結合し、明るい緑色の蛍光が生成されます。DNAに結合する場合の色素の蛍光発光は最大530 nmで、標準的なFITCフィルターセットを使用して観察できます。

使用の提案
• ほとんどの場合、アポトーシスを起こしている細胞の明るい核染色には2滴/mlとインキュベーション時間30分で十分ですが、一部の細胞タイプ、条件、およびアプリケーションには最適化が必要な場合があります。このような場合は、最適な染色強度が得られるまで、加える量を増減します。
•必要に応じて、CellEvent™カスパーゼ-3/7 Green ReadyProbes™試薬を赤色または遠赤色の核染色剤と組み合わせて、総細胞数を測定します。
•CellEvent™カスパーゼ-3/7 Green色素は最大502 nmで励起され、最大530 nmで発光されます。標準のGFPおよびFITCフィルターを介して検出されます。

研究用にのみ使用できます。診断用には使用いただけません。

色Green

適合する細胞哺乳類細胞

励起／発光502/530

使用対象（アプリケーション）セルイメージング

使用対象 (装置)共焦点顕微鏡、Floid™細胞イメージングシステム、蛍光顕微鏡、フローサイトメーター、Floid™細胞イメージングシステム

標識タイプその他の標識または色素

製品ラインCellEvent、ReadyProbes

製品タイプCaspase 3/7試薬

数量1.75 mL

検出法蛍光

フォーマットドロッパーボトル

Unit SizeEach

組成および保存条件

1×1.75 mLのドロッパーボトル

≦25℃で保存してください。

よくあるご質問（FAQ）

I want to use your CellEvent Caspase-3/7 Green detection reagent for studying apoptosis in live cells, but for 96-well microplate instead of microscopy. Will it work? Can I multiplex it with a cell vitality dye?

Yes, on both accounts. We have demonstrated that it works for microplates, and we have multiplexed the dye with PrestoBlue Cell Viability Reagent. Some microplates may be less sensitive than microscopic analysis, so sensitivity may not be the same for the CellEvent reagent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study apoptosis using an Annexin V conjugate, but with adherent cells via microscopy instead of flow cytometry. Can this be done?

It has been done, but we don‘t recommend it. Both healthy cells and apoptotic cells possess phosphatidylserine on the cell surface, which can be detected with Annexin V, but apoptotic cells have significantly more of it. You can easily tell the difference between these two populations with flow cytometry, because flow cytometers are more sensitive and have a higher throughput. But with a microscope, you cannot always tell the difference, especially for adherent cells. Instead, for microscopy, we recommend a different technique, such as detecting caspases with CellEvent Caspase Detection Reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the recommended storage conditions for the CellEvent Caspase-3/7 Green ReadyProbes Reagent (Cat. No. R37111)?

The recommended storage conditions for CellEvent Caspase-3/7 Green ReadyProbes Reagent (Cat. No. R37111) is between 4 and 25 degrees C or room temperature.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long can I measure the Cell Event Caspase-3/7 Detection Reagents (Cat. Nos. C10432, C10433, C10434, C10423, C10723, R37111) signal for the kinetic assay?

We have tested Caspase 3/7 Green Detection Reagents (Cat. Nos. C10432, C10433, C10434, C10423, C10723, R37111) for up to 48 hours and observed no toxicity or stability issues with the reagent. If the samples are fixed at the end of the time course the signal will be retained for days. For live cells, apoptotic cells will round up and float away before the loss of fluorescence signal from CellEvent.

The following reference reports a visible signal for up to 72 hrs:

Sambi M, Samuel V, Qorri B, Haq S, Burov SV, Markvicheva E, Harless W, Szewczuk MR. A Triple Combination of Metformin, Acetylsalicylic Acid, and Oseltamivir Phosphate Impacts Tumour Spheroid Viability and Upends Chemoresistance in Triple-Negative Breast Cancer. Drug Des Devel Ther. 2020 May 25;14:1995-2019. doi: 10.2147/DDDT.S242514. PMID: 32546966; PMCID: PMC7260544.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent FAQs

引用および参考文献 (29)

引用および参考文献

Abstract

SHOX triggers the lysosomal pathway of apoptosis via oxidative stress.

Authors:Hristov G, Marttila T, Durand C, Niesler B, Rappold GA, Marchini A,

Journal:

PubMed ID:24186869

The SHOX gene encodes for a transcription factor important for normal bone development. Mutations in the gene are associated with idiopathic short stature and are responsible for the growth failure and skeletal defects found in the majority of patients with Léri-Weill dyschondrosteosis (LWD) and Langer mesomelic dysplasia. SHOX is expressed ... More

Disruption of mitochondrial quality control genes promotes caspase-resistant cell survival following apoptotic stimuli.

Authors:

Journal:J Biol Chem

PubMed ID:35304098

Design and synthesis of novel 1-phenyl-3-(5-(pyrimidin-4-ylthio)-1,3,4-thiadiazol- 2-yl)urea derivatives with potent anti-CML activity throughout PI3K/AKT signaling pathway.

Authors:Li W, Chu J, Fan T, Zhang W, Yao M, Ning Z, Wang M, Sun J, Zhao X, Wen A

Journal:Bioorg Med Chem Lett

PubMed ID:31097376

'In this investigation, a series of 1-phenyl-3-(5-(pyrimidin-4-ylthio)-1,3,4- thiadiazol-2-yl)urea receptor tyrosine kinase inhibitors were synthesized by a simple and efficient structure-based design. Structure-activity relationship (SAR) analysis of these compounds based on cellular assays led to the discovery of a number of compounds that showed potent activity against human chronic myeloid leukemia ... More

SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial ?? in neuroblastoma cells.

Authors:Tanpure S, Boyineini J, Gnanamony M, Antony R, Fernández KS, Libes J, Lin J, Pinson D, Joseph PA, Gondi CS

Journal:Oncol Lett

PubMed ID:28599461

'Neuroblastoma is the cause of >15% of cancer-associated mortality in children in the USA. Despite aggressive treatment regimens, the long-term survival rate for these children remains at <40%. The current study demonstrates that secreted protein acidic and rich in cysteine (SPARC) suppresses radiation-induced expression of heat shock protein 27 (HSP27)' ... More

The Development of a Nano-based Approach to Alleviate Cisplatin-Induced Ototoxicity.

Authors:Kayyali MN, Ramsey AJ, Higbee-Dempsey EM, Yan L, O'Malley BW, Tsourkas A, Li D

Journal:J Assoc Res Otolaryngol

PubMed ID:29349595

'Cisplatin-induced hearing loss is experienced by a high percentage of patients with squamous cell carcinoma undergoing cisplatin chemotherapy. A novel nano-construct capable of sequestering extracellular cisplatin was developed to combat this problem. The nano-construct consisted of superparamagnetic iron oxide nanoparticles (SPIONs) entrapped within polymeric micelles, which were formed from a ... More

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