SNARF™-4F 5-(and-6)-Carboxylic Acid, Acetoxymethyl Ester, Acetate - Special Packaging

Citations & References (6)

Invitrogen™

SNARF™-4F 5-(and-6)-Carboxylic Acid, Acetoxymethyl Ester, Acetate - Special Packaging

細胞透過性レシオメトリックpHインジケーターSNARF™-4F 5-（および-6）-カルボン酸、アセトオキシメチルエステル、酢酸塩（約6.4のpKa）は、酸性および塩基性条件下で、それぞれ黄橙色から深紅色へとpH依存的に有意な発光シフトを示します詳細を見る

製品番号（カタログ番号）	数量
S23921	20 x 50 μg

製品番号（カタログ番号） S23921

価格（JPY）

116,900

お問い合わせください ›

20 x 50 μg

細胞透過性レシオメトリックpHインジケーターSNARF™-4F 5-（および-6）-カルボン酸、アセトオキシメチルエステル、酢酸塩（約6.4のpK_a）は、酸性および塩基性条件下で、それぞれ黄橙色から深紅色へとpH依存的に有意な発光シフトを示します。このpH依存性により、2つの発光波長（通常580 nmおよび640 nm）における色素からの蛍光強度比をpHの定量測定に使用できます。

カルシウム、カリウム、pH、膜電位インジケータなどのイオンインジケータの詳細をご覧ください›

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

染色剤タイプ蛍光色素ベース

インジケーターpHインジケータ

数量20 x 50 μg

出荷条件室温

使用対象 (装置)蛍光顕微鏡, フローサイトメーター, マイクロプレートリーダー

製品ラインSNARF

製品タイプpHインジケータ

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存。

よくあるご質問（FAQ）

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (6)

引用および参考文献

Abstract

Fluorescent indicators for intracellular pH.

Authors:Han J, Burgess K,

Journal:Chem Rev

PubMed ID:19831417

This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980. ... More

Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.

Authors:Liu J, Diwu Z, Leung WY

Journal:Bioorg Med Chem Lett

PubMed ID:11677123

Two new fluorinated benzo[c]xanthene dyes were synthesized by reaction of fluorinated 1,6-dihydroxynaphthalenes with 2,4- (and 2,5)-dicarboxy-3'-dimethylamino-2'-hydroxybenzophenone. The two critical fluorinated 1,6-dihydroxynaphthalene intermediates were prepared via a regioselective route. The fluorinated benzo[c]xanthene dyes exhibit desired lower pK(a) values (6.4 and 7.2, respectively) than their parent compound (pK(a)=7.5) while the pH-dependent dual-emission ... More

Tissue-specific regulation of sodium/proton exchanger isoform 3 activity in Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) null mice. cAMP inhibition is differentially dependent on NHERF1 and exchange protein directly activated by cAMP in ileum versus proximal tubule.

Authors:Murtazina R, Kovbasnjuk O, Zachos NC, Li X, Chen Y, Hubbard A, Hogema BM, Steplock D, Seidler U, Hoque KM, Tse CM, De Jonge HR, Weinman EJ, Donowitz M,

Journal:J Biol Chem

PubMed ID:17580307

The multi-PDZ domain containing protein Na(+)/H(+) Exchanger Regulatory Factor 1 (NHERF1) binds to Na(+)/H(+) exchanger 3 (NHE3) and is associated with the brush border (BB) membrane of murine kidney and small intestine. Although studies in BB isolated from kidney cortex of wild type and NHERF1(-/-) mice have shown that NHERF1 ... More

Intracellular pH distribution in Saccharomyces cerevisiae cell populations, analyzed by flow cytometry.

Authors:Valli M, Sauer M, Branduardi P, Borth N, Porro D, Mattanovich D

Journal:Appl Environ Microbiol

PubMed ID:15746355

Intracellular pH has an important role in the maintenance of the normal functions of yeast cells. The ability of the cell to maintain this pH homeostasis also in response to environmental changes has gained more and more interest in both basic and applied research. In this study we describe a ... More

pH-Dependence of extrinsic and intrinsic H(+)-ion mobility in the rat ventricular myocyte, investigated using flash photolysis of a caged-H(+) compound.

Authors:Swietach P, Spitzer KW, Vaughan-Jones RD

Journal:Biophys J

PubMed ID:17056723

Passive H(+)-ion mobility within eukaryotic cells is low, due to H(+)-ion binding to cytoplasmic buffers. A localized intracellular acidosis can therefore persist for seconds or even minutes. Because H(+)-ions modulate so many biological processes, spatial intracellular pH (pH(i))-regulation becomes important for coordinating cellular activity. We have investigated spatial pH(i)-regulation in ... More

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