SNARF™-5F 5-(and-6)-Carboxylic Acid

Citations & References (6)

Invitrogen™

SNARF™-5F 5-(and-6)-Carboxylic Acid

細胞不透過性レシオメトリックpHインジケーターSNARF™-5F 5-（および-6）-カルボン酸（約7.2のpKa）は、酸性および塩基性条件下で、それぞれ黄橙色から深紅色へとpH依存的に有意な発光シフトを示します。このpH依存性により、2つの発光波長詳細を見る

製品番号（カタログ番号）	数量
S23922 または、製品番号S-23922	1 mg

製品番号（カタログ番号） S23922

または、製品番号S-23922

価格（JPY）

88,000

お問い合わせください ›

細胞不透過性レシオメトリックpHインジケーターSNARF™-5F 5-（および-6）-カルボン酸（約7.2のpK_a）は、酸性および塩基性条件下で、それぞれ黄橙色から深紅色へとpH依存的に有意な発光シフトを示します。このpH依存性により、2つの発光波長（通常580 nmおよび640 nm）における色素からの蛍光強度比をpHの定量測定に使用できます。

カルシウム、カリウム、pH、膜電位インジケータなどのイオンインジケータの詳細をご覧ください›

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

染色剤タイプ蛍光色素ベース

インジケーターpHインジケータ

数量1 mg

出荷条件室温

使用対象 (装置)蛍光顕微鏡, フローサイトメーター, マイクロプレートリーダー

製品ラインSNARF

製品タイプpHインジケータ

Unit SizeEach

組成および保存条件

室温で保存し、光から保護します。

引用および参考文献 (6)

引用および参考文献

Abstract

Characterization of dual-wavelength seminaphthofluorescein and seminapthorhodafluor dyes for pH sensing under high hydrostatic pressures.

Authors:Salerno M, Ajimo JJ, Dudley JA, Binzel K, Urayama P

Journal:Anal Biochem

PubMed ID:17274941

'Hydrostatic pressure is an important physical parameter in biology, with pressures in the few-hundred-atm range having significant effects on cellular morphology, metabolism, and viability. To ensure valid results when studying pressure effects using fluorescence spectroscopy and imaging methods, metabolic probes need to be characterized for high-pressure use. Of interest is ... More

Fluorescent indicators for intracellular pH.

Authors:Han J, Burgess K,

Journal:Chem Rev

PubMed ID:19831417

This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980. ... More

Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.

Authors:Liu J, Diwu Z, Leung WY

Journal:Bioorg Med Chem Lett

PubMed ID:11677123

Two new fluorinated benzo[c]xanthene dyes were synthesized by reaction of fluorinated 1,6-dihydroxynaphthalenes with 2,4- (and 2,5)-dicarboxy-3'-dimethylamino-2'-hydroxybenzophenone. The two critical fluorinated 1,6-dihydroxynaphthalene intermediates were prepared via a regioselective route. The fluorinated benzo[c]xanthene dyes exhibit desired lower pK(a) values (6.4 and 7.2, respectively) than their parent compound (pK(a)=7.5) while the pH-dependent dual-emission ... More

Using LysoSensor Yellow/Blue DND-160 to sense acidic pH under high hydrostatic pressures.

Authors:DePedro HM, Urayama P,

Journal:Anal Biochem

PubMed ID:18976626

LysoSensor Yellow/Blue DND-160, a dual-wavelength fluorophore commonly used for sensing pH in acidic organelles, possesses solvatochromic behavior believed to originate from an intramolecular charge transfer (ICT). Given this, we investigated whether DND-160 can be used for acidic pH sensing under hydrostatic pressures up to 510 atm, a range suitable for ... More

The chloride transporter Na(+)-K(+)-Cl- cotransporter isoform-1 contributes to intracellular chloride increases after in vitro ischemia.

Authors:Pond BB, Berglund K, Kuner T, Feng G, Augustine GJ, Schwartz-Bloom RD

Journal:J Neurosci

PubMed ID:16452663

Ischemic episodes in the CNS cause significant disturbances in neuronal ionic homeostasis. To directly measure changes in intracellular Cl- concentration ([Cl-]i) during and after ischemia, we used Clomeleon, a novel ratiometric optical indicator for Cl-. Hippocampal slices from adult transgenic mice expressing Clomeleon in hippocampal neurons were subjected to 8 ... More

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